| 金针菇固体发酵菌丝体次级代谢产物分离鉴定 |
| Isolation and identification of secondary metabolites from the solid culture of Flammulina velutipes |
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| 中文关键词:食用菌，金针菇，倍半萜，结构鉴定，抗菌活性 |
| 英文关键词:edible mushroom, Flammulina velutipes, sesquiterpenoids, structural elucidation, antimicrobial activity |
| 基金项目:国家自然科学基金（No. 31000036）；国家重点基础研究发展计划项目（No. 2009CB522300）；中国科学院院方向性项目（No. KSCX2-YW-G-074-04） |
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| 中文摘要: |
| 采用硅胶柱色谱、ODS柱色谱、Sephadex LH-20柱色谱、HPLC等分离方法对金针菇大米发酵乙酸乙酯提取物进行分离，根据理化性质和波谱数据鉴定化合物结构：鉴定了4个倍半萜，包括1个新的桉叶烷型倍半萜，3个侧柏烷型倍半萜，分别是flamvelutpenol A(1)，aquaticol(2)，enokipodin C(3)，limacellone(4)。并通过与Rh2(OCOCF3)4络合的方法确定了新化合物flamvelutpenol A(1)和limacellone(4)的绝对构型。其中化合物3具有较好的抗菌活性，对耐甲氧西林金黄色葡萄球菌和枯草芽孢杆菌的MIC值分别为12.5mg/L，25mg/L。且化合物1－4均是首次从该种真菌中分离得到。 |
| 英文摘要: |
| The ethyl acetate extracts of the solid culture of Flammulina velutipes was subjected to silica gel, ODS, sephadex LH-20 column chromatography and HPLC prepration. Four sesquiterpenoids have been isolated from the mycelial culture of this mushroom. One novel eudesmane-type sesquiterpenoid together with three known cuparene-type sesquiterpenoids were obtained, including flamvelutpenol A (1), aquaticol (2), enokipodin C (3), and limacellone (4). The absolute configuration of 1 and 4 were determined by forming complex with [Rh2(OCOCF3)4] and then applying the bulkiness rule. Compounds 3 showed strong antibacterial activity against methicillin-resistant Staphylococcus aureus and Bacillus subtilis with MIC value 12.5mg/L and 25mg/L. Compounds 1－4 were isolated from the fungus for the first time. |
| 王雅琪,杨晓丽,宝丽,高昊,姚新生,刘宏伟. 金针菇固体发酵菌丝体次级代谢产物分离鉴定[J]. 菌物学报, 2012, 31(1): 127-132 |
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