| 红菇属两种凝集素的分离纯化与比较研究 |
| Purification and comparison of two lectins from the genus Russula |
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| 中文关键词:大白菇，美丽红菇，凝集素，纯化，抗肿瘤 |
| 英文关键词:Russula delica, Russula lepida, lectin, purification, anti-tumor |
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| 摘要点击次数: 1948 |
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| 中文摘要: |
| 以红菇属大白菇Russula delica和美丽红菇Russula lepida子实体为材料，利用离子交换柱层析、凝胶过滤层析的手段，分离获得新的凝集素RDL和RLL。结合凝胶过滤层析和SDS-PAGE的手段，确定RDL和RLL分别是分子量为60kDa和32kDa的双亚基蛋白，其N-末端部分氨基酸序列分别为GLKLAKQFAL和VWYIVAIKTDVPRTT。性质研究表明，RDL在20－70℃、低于25mmol/L HCl或12.5mmol/L NaOH下稳定，其凝集活性可以被邻硝基苯酚-β-D呋喃型半乳糖苷（25mmol/L）和菊糖（50mmol/L）所抑制；RDL具有抑制人肝癌Hep G2和人乳腺癌MCF7细胞增殖以及HIV-1反转录酶（RT）的活性，其半抑制浓度IC50分别为0.88μmol/L、0.52μmol/L和0.26μmol/L。RLL在20－70℃、低于12.5mmol/L HCl或NaOH下稳定，其凝集活性可以被菊糖（25mmol/L）和邻硝基苯酚-β-D呋喃型半乳糖苷（100mmol/L）所抑制；RDL具有抑制人肝癌Hep G2和人乳腺癌MCF7细胞增殖的活性，其半抑制浓度IC50分别为1.60μmol/L和0.90μmol/L，但不具有抑制HIV-1 RT的活性。 |
| 英文摘要: |
| Two novel lectins RDL and RLL were purified from fruiting bodies of Russula delica and Russula lepida using ion exchange chromatography and gel filtration. Based on the results of gel filtration and SDS-PAGE, both RDL and RLL were dimeric proteins with a molecular mass of 60kDa and 32kDa, respectively. The N-terminal amino acid sequences of RDL and RLL were determined to be GLKLAKQFAL and VWYIVAIKTDVPRTT, respectively. RDL was stable in temperature range of 20－70℃, HCl solution up to 25mmol/L, or NaOH solution up to 12.5mmol/L, but was inhibited by o-Nitrophenyl-β-D-galactopyranoside (25mmol/L) and inulin (50mmol/L). RDL manifested antiproliferative activity toward tumor cell lines hepatoma Hep G2 and mastocarinoma MCF7, and inhibitory activity toward HIV-1 reverse transcriptase (RT), with IC50 values of 0.88μmol/L, 0.52μmol/L, and 0.26μmol/L, respectively. RLL was stable in temperature range of 20－70℃, HCl and NaOH solutions up to 12.5mmol/L, but was inhibited by inulin (25mmol/L) and o-Nitrophenyl- β-D-galactopyranoside (100mmol/L). It showed antiproliferative activity toward Hep G2 and MCF7, with IC50 values of 1.60μmol/L and 0.90μmol/L, respectively, but was lacking in HIV-1 RT inhibitory activity. |
| 张国庆,陈青君,赵爽,王守现,王贺祥. 红菇属两种凝集素的分离纯化与比较研究[J]. 菌物学报, 2012, 31(1): 110-118 |
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