Anti-oxidation activity of ethanol extracts from natural thalli of lichens |
Anti-oxidation activity of ethanol extracts from natural thalli of lichens |
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中文关键词: |
英文关键词:anti-oxidation activity, natural thalli, lichen, screening |
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作者 | 单位 | Kojiro HARA | Faculty of Bioresource Sciences, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita City 010-0195, Japan | Marie ENDO | Faculty of Bioresource Sciences, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita City 010-0195, Japan | Hiroko KAWAKAMI | Faculty of Bioresource Sciences, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita City 010-0195, Japan | Masashi KOMINE | Faculty of Bioresource Sciences, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita City 010-0195, Japan | Yoshikazu YAMAMOTO | Faculty of Bioresource Sciences, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita City 010-0195, Japan |
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中文摘要: |
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英文摘要: |
Screening test on anti-oxidation activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) was performed for 99 ethanol extracts of 85 species of natural thalli of lichens in order to find novel anti-oxidation compounds. The 17 extracts of natural thalli showed high anti-oxidation activity. Among them, the activities of extracts from Hypogymnia vittata, Peltigera aphthosa, Nephromopsis ornata, Pseudevernia furfuracea, Cladonia vulcani and Peltigera elizabethae were higher. Extracts of Peltigera spp. showed higher activity than those of other genera. The ethanol extract of P. aphthosa had been separated into ethyl acetate-soluble and water-soluble fractions. Two anti-oxidative spots were found only in the water-soluble fractions by thin-layer chromatography. The compound in the lower spot had the same Rf value, UV spectrum, and color as authentic solorinine that was previously found as a unique quaternary ammonium compound from Peltigera spp. We now report that the hydrophilic lichen substance, solorinine showed a nearly same anti-oxidation activity (EC50=120μmol/Lol/L) as standard antioxidant Trolox (EC50=150μmol/L). |
Kojiro HARA,Marie ENDO,Hiroko KAWAKAMI,Masashi KOMINE,Yoshikazu YAMAMOTO. Anti-oxidation activity of ethanol extracts from natural thalli of lichens[J]. 菌物学报, 2011, 30(6): 950-954 |
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