| ITS作为粒毛盘菌属DNA条形码的探索 |
| Evaluation of ITS region as a possible DNA barcode for the genus Lachnum (Helotiales) |
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| 中文关键词:条形码间隔区，种内与种间变异，PCR扩增及测序成功率 |
| 英文关键词:barcoding gap, intra- and inter-specific variation, PCR and sequencing success rate |
| 基金项目:National Natural Science Foundation of China (No. 31070015) and the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KSCX2-EW-J-6). |
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| 中文摘要: |
| 以种内与种间差异以及PCR扩增和测序成功率为评价DNA条形码的重要指标，探索ITS序列作为粒毛盘菌属DNA条形码的可行性。结果表明，ITS成功区分了研究涉及的22个种，其PCR扩增和测序成功率为100%，该片段有望成为该属区分物种的DNA条形码。 |
| 英文摘要: |
| The feasibility of ITS as a DNA barcode for the genus Lachnum was investigated. Intra- and inter-specific sequence divergences and easiness of sequence acquisition were considered as important criteria to estimate the barcode marker. The results revealed that ITS showed appropriate intra- and inter-specific variations, was able to separate the investigated 22 species of the genus, and was readily PCR amplified and sequenced. We suggest to use ITS as the potential DNA barcode for the genus. |
| 赵鹏,庄文颖. ITS作为粒毛盘菌属DNA条形码的探索[J]. 菌物学报, 2011, 30(6): 932-937 |
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