| 双向核迁移在香菇遗传和育种中的应用研究 |
| The application of the ‘bidirectional haploid nuclei migration’ in breeding and genetics of Lentinula edodes |
| |
| 中文关键词:单-单杂交，双-单杂交，布勒现象 |
| 英文关键词:monokaryon mating, dikaryon-monokaryon mating, Buller phenomenon |
| 基金项目:福建省科技重大专项（No. 2008SZ0002）；公益类科研院所专项项目（No. 2009R10009-1）；农业科技重点项目（No. 2009N0028） |
|
| 摘要点击次数: 2333 |
| 全文下载次数: 1163 |
| 中文摘要: |
| 在来源于香菇Lentinula edodes不同双核菌株的单核菌株间进行单-单杂交，并利用双向核迁移现象获得两对双核菌株。之后采用挑取菌落尖端单根菌丝的方法纯化所获得的双核菌株并通过双-单杂交进行单向核迁移，进一步纯化所获得的双核菌株。从菌落形态、显微镜镜检、出菇实验和分子标记等4个方面对两对配对单核和所获得的双核菌株进行分析和鉴定。结果表明：（1）通过双向核迁移的方法可以获得具有两个相同细胞核和不同受体细胞质的香菇双核菌株；（2）选择不同的受体细胞会对双核菌丝的菌落形态和出菇期产生明显的影响，表明香菇的 |
| 英文摘要: |
| Monokaryon mating tests between different strains of Lentinula edodes were carried out, and two pair dikaryotic strains were obtained by utilizing the mechanism of ‘bidirectional haploid nuclei migration’. The strains were purified by isolation of single hyphal tips of dikaryotic strains and making use of ‘Buller phenomenon’. Two pairs of monokaryotic and dikaryotic strains were analyzed by observations of colony shapes, microscopic examination, fruiting tests, and molecular markers. The results showed that the strains of L. edodes consisting of the same nuclei but different cytoplasm could be obtained, different strains with accepted cytoplasm of dikaryotic mycelia appeared to have different colony shapes and fruiting-date. The data indicated that the factors in cytoplasm were probably responsible for these phenomena. |
| 刘靖宇,刘新锐,邓优锦,刘芳,谢宝贵. 双向核迁移在香菇遗传和育种中的应用研究[J]. 菌物学报, 2011, 30(5): 774-781 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|