| 树状多节孢酸性磷酸酶的分离纯化与性质研究 |
| Purification and characterization of an acid phosphatase from Nodulisporium sylviforme |
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| 中文关键词:树状多节孢，酸性磷酸酶，性质，酶活性 |
| 英文关键词:Nodulisporium sylviforme, acid phosphatase, characteristics, enzyme activity |
| 基金项目:北京市科委项目（No. Z101105002510003）；北京市属高等学校人才强教项目（No. PHR200907136） |
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| 中文摘要: |
| 树状多节孢Nodulisporium sylviforme是从东北红豆杉Taxus cuspidata分离、可产生紫杉醇的内生真菌。研究以树状多节孢为材料，利用液体发酵手段获得菌丝体，通过CM-cellulose阴离子交换柱层析、Q-Sepharose阳离子交换柱层析和FPLC凝胶过滤层析（Superdex 75），获得纯化的树状多节孢酸性磷酸酶蛋白（Nod-ACP）。结合FPLC和SDS-PAGE分析，判定该磷酸酶为分子量44kDa单亚基蛋白。酶学性质研究表明，其最适pH值为3.0，最适温度为58℃。6 |
| 英文摘要: |
| A taxol-producing endophytic fungus Nodulisporium sylviforme was isolated from Taxus cuspidata. In this study, a novel acid phosphatase was purified from the fungus by using a schedule of fermentation, ion exchange chromatography on CM-cellulose, Q-Sepharose and gel filtration by fast protein liquid chromatography on Superdex 75. The phosphatase was a monomeric protein with a molecular mass of 44kDa. Its optimum pH was 3.0 and optimum temperature 58℃. The activity of the phosphatase was enhanced by 30%－50% in the presence of Ca2+, Mn2+, Mg2+ and Zn2+ cations with the concentrations of 6.25－50mmol/L, and was reduced in the presence of Cu2+, Al3+ and Fe3+ cations. Fe2+ increased the enzyme activity at the concentration lower than 25mmol/L, but inhibited the enzyme activity completely at the concentration more than 50mmol/L. |
| 张国庆,王有志,王守现,王贺祥,陈青君. 树状多节孢酸性磷酸酶的分离纯化与性质研究[J]. 菌物学报, 2011, 30(5): 753-759 |
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