| 洪湖沉积物中真菌群落结构的垂直分布 |
| Vertical distribution of fungal communities in Honghu Lake sediment |
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| 中文关键词:内转录间隔区（ITS），变性梯度凝胶电泳（DGGE），子囊菌门，担子菌门 |
| 英文关键词:internal transcribed spacer (ITS), denaturing gradient gel electrophoresis (DGGE), Ascomycota, Basidiomycota |
| 基金项目:中央高校基本科研业务费专项资金（No. 2009PY013） |
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| 中文摘要: |
| 湖泊沉积物中真菌的类群及其作用所知甚少。以长江中上游代表性湖泊洪湖为研究对象，从湖心截取了一个72cm长的沉积柱，通过rDNA内转录间隔区（ITS）的变性梯度凝胶电泳（DGGE），对洪湖沉积物中真菌群落结构的垂直变化进行了分析。结果显示，DGGE条带以14－18cm层次为过渡，在2－14cm和14－72cm之间存在一定差异。Shannon-Weaver index（H′）在上下层之间有波动。聚类分析上层2－18cm和中下层18－72cm分别以不同的小类聚在一起。表明14－18cm以上和以下的类群存在差异， |
| 英文摘要: |
| The fungal communities and their function in lake sediments are poorly understood. In this study, Honghu Lake, a representative lake at the middle and upper reaches of the Yangtze River, was selected as the sampling place. A 72cm sediment core was obtained from the center of the lake to investigate the vertical changes of fungal community structures by denaturing gradient gel electrophoresis (DGGE) analysis of internal transcribed spacer (ITS) of rDNA. DGGE profiles had some differences between the samples from the depth regions of 2－14cm and 14－72cm with the 14－18cm region as the transition point. The Shannon-Weaver index (H′) fluctuated at different depths. Clustering analysis showed that the depth regions of upper 2－18cm and 18－72cm were clustered into separate clades with different subclasses. The results indicated that the fungal communities were different between the depth regions of 2－14cm and 14－72cm, i.e. the fungal groups changed obviously with depths. The fungal communities mainly included the species of Ascomycota and Basidiomycota, and the species of Ascomycota dominated in the sediment, which accounted for 70% of the detected groups. The ascomycotes were mainly in the subphyla of Saccharomycotina and Pezizomycotina. The Basidiomycota phylum included Heterobasidiomycetes, Ustilaginomycetes and unclassified Basidiomycota. |
| 赵彦玲,郑世学,方治伟,喻子牛. 洪湖沉积物中真菌群落结构的垂直分布[J]. 菌物学报, 2011, 30(5): 721-728 |
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