菌物学报

法国蜜环菌Armillaria gallica菌株遗传多样性的ISSR分析

Genetic diversity of Armillaria gallica revealed by ISSR analysis

英文关键词:genetic relationship, cluster analysis, geographical isolation

基金项目:浙江省自然科学基金（No. Y3090409）；哈尔滨市青年科学基金（No. 2002AFQXJ006）

作者	单位
孙立夫	绍兴文理学院生命科学学院绍兴 312000
裴克全	中国科学院植物研究所生态中心北京 100093
张艳华	绍兴文理学院生命科学学院绍兴 312000
赵俊	国家林业局森林病虫害防治总站沈阳 110034
杨国亭	东北林业大学林学院哈尔滨 150040
秦国夫	国家林业局森林病虫害防治总站沈阳 110034
宋玉双	国家林业局森林病虫害防治总站沈阳 110034
宋瑞清	东北林业大学林学院哈尔滨 150040

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中文摘要:

在中国东北地区共采集到53个法国蜜环菌Armillaria gallica菌株，用ISSR（Inter-Simple Sequence Repeat）标记技术对这些菌株进行遗传多样性分析。用6个ISSR引物扩增所得条带表明，ISSR标记在蜜环菌中存在较高的多态性；亲缘关系树状图表明，有3个菌株遗传分化明显；其余50个分别来自3个不同地理居群的菌株聚成一类，亲缘关系较近，没有表现出地理隔离。

英文摘要:

Fifty-three isolates of Armillaria gallica were collected in the Northeast of China. The genetic diversity of these isolates was revealed by ISSR analysis. Polymorphic bands were generated with 6 primers indicated that high diversity was conserved in the Chinese A. gallica isolates. The dendrogram of genetic relationships demonstrated that 3 isolates of China had generated distinct genetic differentiation. The other 50 isolates which were sampled from 3 different geographic populations were mixed into one cluster, and showed a closer genetic relationship without geographical isolation.

孙立夫,裴克全,张艳华,赵俊,杨国亭,秦国夫,宋玉双,宋瑞清. 法国蜜环菌Armillaria gallica菌株遗传多样性的ISSR分析[J]. 菌物学报, 2011, 30(5): 686-694

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