| CO1在侧耳属物种快速鉴定中的应用 |
| Application of CO1 for rapid identification of Pleurotus species |
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| 中文关键词:内转录间隔区，两轮PCR，种特异引物 |
| 英文关键词:ITS, two-step PCR, species-specific primer |
| 基金项目:中央级公益性科研院所基本科研业务费专项（No. 2009-13） |
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| 中文摘要: |
| 以侧耳属Pleurotus15个种的15个菌株为材料，根据GenBank上侧耳属细胞色素c氧化酶亚基Ⅰ基因（cytochrome c oxidase subunit 1 gene，CO1）序列信息，设计引物CO332F、CO332R，进行第一轮PCR扩增，结果显示所有菌株都能得到单一条带，根据条带大小，15个菌株可分为4组。随后针对每个种设计特异性引物，进行第二轮PCR扩增，结果显示每个菌株只有在自己特异的引物中出现目的条带。通过两轮扩增，根据扩增条带的大小和有无，即可对15个种进行快速鉴定。 |
| 英文摘要: |
| 15 strains belonging to 15 species of Pleurotus were used. Primer CO332F and CO332R were designed by the cytochrome c oxidase subunit 1 gene (CO1) sequence of Pleurotus downloaded from GenBank. Results of the first-step PCR amplification showed that all the strains can get one band. The 15 strains were divided into four groups by the band size. Species-specific primers were designed accordingly. Results of the second-step PCR amplification showed that each species can get one band by the species-specific primers. In conclusion, the 15 species of Pleurotus can be identified by two-step PCR in accordance with the band size and the presence or absence of the band. |
| 宋驰,陈强,徐璟煜,张金霞,边银丙,黄晨阳. CO1在侧耳属物种快速鉴定中的应用[J]. 菌物学报, 2011, 30(4): 663-668 |
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