| 相似蜂海绵相关真菌杂色曲霉F62活性代谢产物研究 |
| The bioactive metabolites of Aspergillus versicolor F62 isolated from Haliclona simulans |
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| 中文关键词:海绵相关真菌，杂色曲霉，次生代谢产物，活性 |
| 英文关键词:sponge-associated fungus, Aspergillus versicolor, secondary metabolites, activity |
| 基金项目:国家“十一五”科技重大专项“综合性新药研究开发技术大平台”（No. 2009ZX09301-003-4-1） |
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| 中文摘要: |
| 研究了1株相似蜂海绵相关真菌F62的活性代谢产物，经分子系统学分析表明该真菌属于杂色曲霉。将F62菌株用大米固体培养基在室温（约25℃）下静置培养45d，经乙酸乙酯超声萃取得到粗提物。通过硅胶柱层析、Sephadex LH-20凝胶柱层析和HPLC等色谱手段，分离得到了6个化合物。通过核磁共振、质谱等波谱分析手段可鉴定出其结构分别为Alantrypinone（1）、洛伐他汀（2）、甲酯型莫纳克林K（3）、土曲霉酮（4）、土震素B（5）和麦角甾醇（6）。化合物1系首次从该属真菌中分离得到，化合物2－5为首次从该种真菌中分离得到。首次对化合物3的碳谱数据进行报道。初步的药理研究表明，化合物4具有体外抗炎活性。 |
| 英文摘要: |
| A marine fungal strain F62 primarily determined as Aspergillus versicolor was isolated from sponge Haliclona simulans. The sponge-associated fungus was cultivated on the rice solid medium at room temperature (about 25℃) for 45 days. The culture was extracted with ethylacetate and the total extract was purified by means of silica gel, Sephadex LH-20 column chromatography, high performance liquid chromatography (HPLC), and so on. Six compounds were obtained and the structures were determined as alantrypinone (1), lovastatin (2), methylester of lactone ring-opened monacolin K (3), terrein (4), territrems B (5) and ergosterol (6), respectively, based on nuclear magnetic resonance (NMR), mass spectrum (MS) and other spectroscopic determination. Compound 1 and compounds 2－5 were first isolated from the genus Aspergillus and Aspergillus versicolor, respectively. 13C NMR spectral data of compound 3 were provided for the first time. Compound 4 was first reported to have anti-inflammatory activity in vitro. |
| 董世豪,巩婷,朱平. 相似蜂海绵相关真菌杂色曲霉F62活性代谢产物研究[J]. 菌物学报, 2011, 30(4): 636-643 |
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