山野木层孔菌子实体中抑制H22荷瘤小鼠肿瘤的活性成分研究
Antitumor active constituent in fruiting body of Phellinus yamanoi against Hepatoma H22 cell
  
中文关键词:山野木层孔菌,抑瘤活性,白介素-2,肿瘤坏死因子-α
英文关键词:Phellinus yamanoi, antitumor activity, inter leukin-2 (IL-2), tumor necrosis factor-α
基金项目:国家自然科学基金(No. 30870013)
作者单位
梁伟 吉林农业大学中药材学院 长春 130118 
包海鹰 吉林农业大学中药材学院 长春 130118 
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中文摘要:
      采用梯度提取法对山野木层孔菌子实体进行提取,得到石油醚层、甲醇层及水层3 种提取物及石油醚层中获得化合物4,6,8(14),22(23)-四烯-3-酮-麦角甾烷,并采用H22 荷瘤小鼠进行体内抗肿瘤活性研究,以抑瘤率、免疫器官指数、免疫因子为指标检测抗肿瘤活性。结果表明,石油醚高剂量组(100mg/kg)、单体化合物中剂量组(7.5mg/kg)抑制率分别为62.21%、57.67%;脾指数、胸腺指数均高于对照组和环磷酰胺(CTX)组,白介素-2(IL-2)的含量明显高于对照组和环磷酰胺(CTX)组(P<0.01);肿瘤坏死因子-α(TNF-α)含量明显低于对照组(P<0.01)。因此认为上述石油醚提取物和单体化合物对H22荷瘤小鼠肿瘤有抑制作用,并且均能改善小鼠的免疫功能。
英文摘要:
      Three kinds of extracts were made from the fruiting body of Phellinus yamanoi by gradient extraction method using petroleum ether, methanol and water as extracting solution, and 4,6,8(14),22(23)-tetraen-3-one-ergostane was isolated from petroleum ether extract. The anti-tumor activities of the three extracts and the monomer compound against mice Hepatoma H22 were studied in vivo. Inhibition rates of tumor, immune organ indexes and the contents of immune factors were evaluated as indicators. The results showed that the inhibition rates of petroleum ether extract for high dosage group (100mg/kg) and those of monomer compound for middle dosage group (7.5mg/kg) were 62.21% and 57.67%, respectively. Spleen indexes and thymus indexes of petroleum ether extract and monomer compound were higher than those of control group and cyclophosphamide-treated group (CTX) (P<0.01), meanwhile inter leukin-2 (IL-2) level of them was obviously higher than that of the control group and cyclophosphamide-treated group (P<0.01). Tumor necrosis factor-α (TNF-α) level of petroleum ether extract and monomer compound was significantly lower than that of the control group (P<0.01). Hence, the petroleum ether extract and the monomer compound of fruiting body of P. yamanoi inhabit Hepatoma H22 cell of mice, and also can improve mice’s immune function.
梁伟,包海鹰. 山野木层孔菌子实体中抑制H22荷瘤小鼠肿瘤的活性成分研究[J]. 菌物学报, 2011, 30(4): 630-635
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