| 双孢蘑菇基质降解能力退化的差异蛋白质组学分析 |
| Differential proteomics analysis of degeneration on the substrate- decomposing of Agaricus bisporus |
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| 中文关键词:食用菌，蛋白质组，双向电泳，差异蛋白质，质谱分析 |
| 英文关键词:edible fungus, proteome, two-dimensional electrophoresis, differential protein, MALDI-TOF/MS analysis |
| 基金项目:国家自然科学基金（No. 30871769），福建省重大专题（No. 2008SZ0002） |
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| 中文摘要: |
| 对双孢蘑菇CGMCC No. 0214及其基质降解能力退化菌株0214-3、0214-5进行了蛋白质双向电泳（2-DE或2D-PAGE）分析，发现了2个明显的、可重复的差异蛋白质，在退化菌株中分别为上调和下调表达，且2个退化菌株表现一致。根据质谱（MALDI-TOF/MS）分析和数据库检索结果，2个差异蛋白质初步鉴定为肌动蛋白和NADH脱氢酶铁硫蛋白3。 |
| 英文摘要: |
| The technique of two-dimensional electrophoresis (2-DE or 2D-PAGE) was used to analyze the protein expression differences between the wild type Agaricus bisporus CGMCC No. 0214 and its substrate-decomposing ability degenerated strains 0214-3, 0214-5. As a result, two proteins were found to be up- and down-expressed, respectively, with obvious and repeatable profiles in both degenerated strains when grown in a liquid culture. Based on the results of MALDI-TOF/MS analysis and database searching, these two proteins were identified as an actin and a putative NADH dehydrogenase iron-sulfur protein 3, respectively. The results of this study provided a marker on the proteomics level for further study of the molecular mechanism of substrate-decomposing ability degeneration in A. bisporus. |
| 陈美元,王泽生,廖剑华,李洪荣,蔡志欣,谢宝贵. 双孢蘑菇基质降解能力退化的差异蛋白质组学分析[J]. 菌物学报, 2011, 30(3): 508-513 |
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