| 不同成熟期大秃马勃子实体提取物的抑菌活性及其挥发油成分分析 |
| Antibacterial activities and volatile oil component analysis of extracts of Calvatia gigantea fruiting bodies in different maturity period |
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| 中文关键词:大秃马勃，化学成分，GC-MS |
| 英文关键词:Calvatia gigantea, chemical constituent, gas chromatograpby-mass spectrometry |
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| 中文摘要: |
| 对不同成熟期大秃马勃Calvatia gigantea子实体的提取物抑菌活性进行了初步研究，并采用水蒸气蒸馏法提取挥发油，利用GC-MS联用仪对成分进行了分析鉴定，还对挥发油的抑菌活性进行了研究。结果表明，对大肠杆菌ATCC8099和金黄色葡萄球菌ATCC6538抑制作用较强的是未成熟大秃马勃的氯仿提取物，浓度为50mg/mL时抑菌率分别为57.57%和50.12%，MIC值均为12.5mg/mL。不同成熟期的挥发油在成分组成上的主要区别是未成熟子实体的挥发油含有雪松醇、α-石竹烯及石竹烯氧化物，而成熟子 |
| 英文摘要: |
| Antibacterial activity of extracts segregated by different extracting solution from fruiting bodies of Calvatia gigantea of different maturity stage was studied. Volatile oil from C. gigantea was extracted by steam-distillation method, and its components were analyzed by gas chromatography-mass spectrometry (GC-MS). The antibacterial activity of volatile oil was also studied. The results indicated that chloroform extract of immature fruiting body had obvious inhibiting activity to Escherichia coli ATCC8099 and Staphylococcus aureus ATCC6538 at a 50mg/mL concentration level. The inhibition rate was 57.57% and 50.12%, respectively. The MIC values were all at 12.5mg/mL. The main difference of volatile oil composition between immature and mature fruiting body was that volatile oil from immature fruiting body contained cedrol, α-caryophyllene and caryophyllene oxide, but that from mature fruiting body did not. The volatile oil from immature fruiting body performed stronger inhibitory activity to Escherichia coli ATCC8099 and Staphylococcus aureus ATCC6538, and both MIC values were 10.0mg/mL. |
| 游洋,包海鹰. 不同成熟期大秃马勃子实体提取物的抑菌活性及其挥发油成分分析[J]. 菌物学报, 2011, 30(3): 477-485 |
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