| 斑点嗜蓝孢孔菌化学成分、生物活性及水提物荧光猝灭研究 |
| Chemical constituents, biological activities and flourescence quenching of Fomitiporia punctata fruiting body extract |
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| 中文关键词:斑点嗜蓝孢孔菌，化学成分，抗肿瘤活性，抗氧化活性，荧光猝灭 |
| 英文关键词:Fomitiporia punctata, chemical composition, anti-tumor activity, antioxidant activity, fluorescence quenching |
| 基金项目:国家高技术研究发展计划（863计划）（No. 2007AA021506）；江苏省高校自然科学重大基础研究项目（No. 08KJA350001） |
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| 中文摘要: |
| 对斑点嗜蓝孢孔菌乙醇提取物进行分级萃取，利用柱层析技术得到6个单体化合物。经核磁数据比对为β-谷甾醇(1)、麦角甾醇(2)、麦角甾-7,22-二烯-3β-醇(3)、5,8-过氧麦角甾-6,22-二烯-3β-醇(4)、3-乙酰齐墩果酸(5)、白桦脂醇(6)。采用Alamar Blue法检测各有机相及单体化合物对NCI-H460人非小细胞肺癌细胞增殖的影响，用DPPH自由基清除法检测其抗氧化活性。利用荧光光谱及紫外-可见光光谱研究方法在模拟人体生理条件下，研究了斑点嗜蓝孢孔菌水提物与人纤维蛋白原的相互作用，实 |
| 英文摘要: |
| Different polar fractions were extracted by different reagents from the EtOH-extract of Fomitiporia punctata. Six compounds were isolated and identified as β-sitosterol (1), egosterol (2), erogosta 7,22-dien-3β-ol (3), 5,8-epidioxiergosta- 6,22-dien-3β-ol (4), 3-acetoxyoleanolic acid (5) and botulin (6) by the technology of NMR. The anti-tumor activity against NCI-460 was evaluated by Alamar Blue method, and the antioxidant activity was detected by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. The interactions between aqueous extract and human fibrinogen were investigated by UV/vis absorption and fluorescence spectrum methods. The results showed that the process of binding aqueous extract of F. punctata against human fibrinogen was a spontaneous molecular interaction procedure. |
| 袁博,朱峰,陈永强,黄天姿,鞠秀云,王力,冯友建,蒋继宏. 斑点嗜蓝孢孔菌化学成分、生物活性及水提物荧光猝灭研究[J]. 菌物学报, 2011, 30(3): 464-471 |
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