| 短期物理应激对云芝菌丝体SOD活性的影响 |
| Effects of short-term physical stresses on activities of superoxide dismutase (SOD) in Trametes versicolor mycelium |
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| 中文关键词:云芝，超氧化物歧化酶，温度胁迫，超声波，正交试验 |
| 英文关键词:Trametes versicolor, superoxide dismutase, temperature stress, ultrasound wave, orthogonal test |
| 基金项目:深圳职业技术学院重点科技开发项目（No. 01KJb016） |
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| 中文摘要: |
| 选取高低温分别胁迫、协同胁迫以及超声波处理等方式研究了短期物理应激对云芝菌丝体超氧化物歧化酶（SOD）活性的影响。与未作胁迫处理的对照相比，在低温处理中，10℃较之15℃处理SOD活性整体水平有明显提高，10℃处理2h可使SOD活性提高36.2%，延长处理时间导致SOD活性下降；高温协迫中30℃处理效果优于40℃、50℃和60℃，30℃处理4h，SOD相对酶活可提高至26.8%，延长处理时间和提高处理温度均导致SOD活性下降。高低温协同作用L16(2×42)正交试验结果分析表明，30℃处理2h后10℃处理 |
| 英文摘要: |
| The activities of superoxide dismutase (SOD, EC 1.15.1.1) in Trametes versicolor mycelium were investigated under short-term physical stresses including single temperature, high and low synergistic temperature and ultrasound radiation. Compared with mycelium cultivated without stresses, the SOD activity increased by 36.2% at low temperature of 10℃ for 2h, higher than that treated at 15℃. Treated at 30℃ for 4h, the SOD activity increased by 26.8%, higher than that treated at 40℃, 50℃ and 60℃. However, treatments with higher temperature and longer duration caused a constant decrease. The L16(2×42) orthogonal test showed that the SOD activity significantly increased by 54.6% when the mycelium was treated at 30℃ for 2h, followed by at 10℃ for 1.5h, in comparison with that treated at low or high temperature alone. No significant difference was observed under reverse procedure of temperature treatment. The activity of SOD increased by 41.9% when mycelium had been stimulated by ultrasound wave at 38kHz for 40min, but deceased when treated with extended time. These results indicate that the short-term physical stresses including temperature and ultrasound radiation had elevated effects on SOD activity in Trametes versicolor mycelium when suitable treatment conditions were adopted. The combination of high and low synergistic temperature significantly increased the SOD activity, as compared with treatment of single temperature. |
| 黄志立,黄彦君,张丽君,党建章,梁世中. 短期物理应激对云芝菌丝体SOD活性的影响[J]. 菌物学报, 2011, 30(3): 453-458 |
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