| 脉孢菌lca-1基因调控无性产孢及类胡萝卜素的合成 |
| lca-1 regulating conidiation and carotenoid production in Neurospora |
| |
| 中文关键词:类胡萝卜素，粗糙脉孢菌，无性产孢，lca-1 |
| 英文关键词:carotenoid, Neurospora crassa, conidiation, lca-1 |
| 基金项目:国家自然科学基金（No. 31000551，No. 30970127） |
|
| 摘要点击次数: 1523 |
| 全文下载次数: 1071 |
| 中文摘要: |
| 类胡萝卜素是很多生物细胞内重要的抗氧化剂，具有保护细胞免受紫外线伤害的功能。粗糙脉孢菌是少数几个类胡萝卜素合成基因比较清楚的真菌之一，为了深入了解该菌类胡萝卜素合成调控机制，通过对粗糙脉孢菌基因突变体库中6,087株突变体进行筛选，新发现6个基因敲除突变体营养生长正常，但类胡萝卜素的合成降低，其中表型较好的1个突变体，其无性产孢量与类胡萝卜素合成量均明显降低。鉴定发现该突变体所缺失的基因编码一种依赖ATP的染色体重建复合体的ATP酶链ISW1，将该基因命名为lca-1。进一步测定发现lca-1基因的突变导 |
| 英文摘要: |
| Carotenoid is an antioxidative pigment providing protection against UV radiation in fungi. Neurospora crassa is one of a few fungi from which genes participate in carotenoid biosynthesis have been characterized. In order to investigate the regulatory mechanisms of carotenoid biosynthesis in N. crassa, we screened mutants for lower carotenoid production and found 6 belonging to this category from 6,087 gene knockout mutants. Among them, a mutant whose corresponding gene was named as lca-1 in this study was further investigated. lca-1 encodes a ATP-dependent chromatin remodeling complex ATPase chain ISW1. lca-1 deletion resulted in a reduction of 88% in conidial production and a decrease of 81% in carotenoid production. qRT-PCR analysis showed that the expression levels of the four genes (al-1, al-2, fl and eas) related to conidiation and carotenoid biosynthesis were reduced. These results suggest that lca-1 positively regulates conidiation and carotenoid biosynthesis in Neurospora. |
| 何纯,孙宪昀,段碧华. 脉孢菌lca-1基因调控无性产孢及类胡萝卜素的合成[J]. 菌物学报, 2011, 30(3): 435-441 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|