| 黑柄炭角菌的单核苷酸多态性研究 |
| Single nucleotide polymorphisms of Xylaria nigripes |
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| 中文关键词:黑柄炭角菌，分子标记，单倍型，异核体 |
| 英文关键词:Xylaria nigripes, molecular markers, haplotype, heterokaryon |
| 基金项目:Key Scientific Research Fund of Hunan Provincial Education Department (No. 08A044), National Natural Science Foundation of China (No. 30770010), the Program for Excellent Talents in Hunan Normal University (No. 2008-6). |
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| 中文摘要: |
| 以SNPs作为分子标记，对黑柄炭角菌的生活史和遗传多样性进行研究。基于黑柄炭角菌的基因组文库，在两个菌株（X-WC和X-LY）的34个片段（19,680bp）中共发现193个SNPs，SNP发生率为0.981%。193个SNPs位点都有两个可变的核苷酸，其中132个转换，61个颠换，转换与颠换比率为2.16。此外，对两个菌株序列进行比较，发现了41个插入/缺失位点。从34个片段中选择4个片段，分别比较菌株内的SNPs情况。X-WC的菌株内SNP发生率为每个核苷酸1.308%（30/2293），X-LY的菌 |
| 英文摘要: |
| To study the life cycle and genetic diversity of the ascomycete fungus Xylaria nigripes, single nucleotide polymorphisms (SNPs) were used as molecular marks. Based on the genomic library, a total of 193 SNPs were found in 34 fragments with 19,680bp from the two different individuals of X. nigripes, X-WC and X-LY, representing an SNP frequency of 0.981%. Among the 193 SNPs, all the sites had two alternative nucleotides, with 132 transitions and 61 transversions, resulting in a combined transition to transversion ratio of 2.16. The comparisons between the two individuals resulted in identification of 41 indels. Four of the 34 fragments were selected to examine intra-individual SNPs of the two individuals. Individual X-WC had an intra-individual SNP frequency of 1.308% (30/2293) per nucleotide and individual X-LY had a SNP frequency of 1.352% (31/2293) per nucleotide. Thirty-one sites double peak in the sequencing chromatograph indicated that the sclerotia of individual X-LY were heterokaryon. |
| 马橙,陈作红,张平. 黑柄炭角菌的单核苷酸多态性研究[J]. 菌物学报, 2011, 30(3): 426-434 |
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