| 鲍姆木层孔菌多糖对HepG2细胞增殖及侵袭相关能力的抑制作用 |
| Inhibitory effects of Phellinus baumii polysaccharide on the proliferation and invasion of HepG2 cell |
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| 中文关键词:大型真菌，抗肿瘤，细胞周期，侵袭 |
| 英文关键词:macrofungi, antitumor, cell cycle, invasiveness |
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| 中文摘要: |
| 证实了鲍姆木层孔菌多糖对肝癌细胞系HepG2细胞增殖及侵袭的抑制作用。鲍姆木层孔菌提取物经乙醇分级沉淀和DEAE-Sepharoes F.F.离子柱层析以及Sephacryl S-200凝胶柱层析纯化，获得均一多糖PLP60-B1，该多糖具有体外活性。MTT法及流式细胞仪技术证实多糖PLP60-B1通过致使HepG2细胞阻滞于S期而显著抑制HepG2细胞的增殖和细胞集落的形成，且也可显著抑制细胞的粘附及侵袭能力。 |
| 英文摘要: |
| The inhibitory effects of Phellinus baumii polysaccharide PLP60-B1 on the proliferation and invasion of HepG2 cancer cells were investigated. The homogeneous Phellinus baumii polysaccharide PLP60-B1 having promoting effects on lymphocyte proliferation was obtained by ethanol precipitation and purified by DEAE-Sepharose F.F. and Sephacryl S-200. MTT assay and flow cytometry showed that PLP60-B1 inhibited cell proliferation as well as colony formation of HepG2 cells through the S-phase cell cycle arrest, and the homogeneous polysaccharide also demonstrated the remarkably inhibition of adhesion and invasion in HepG2 cells. |
| 王贵宾,董露璐,姬媛媛,相文华,赵敏. 鲍姆木层孔菌多糖对HepG2细胞增殖及侵袭相关能力的抑制作用[J]. 菌物学报, 2011, 30(2): 288-294 |
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