| 海洋真菌帚状弯孢聚壳FS26代谢产物的分离鉴定与抗肿瘤活性研究 |
| Purification, identification and antitumor activities of secondary metabolites from marine fungus Eutypella scoparia FS26 |
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| 中文关键词:海洋真菌，帚状弯孢聚壳，色谱技术，重结晶，细胞松弛素类 |
| 英文关键词:marine fungus, Eutypella scoparia, chromatographic methods, recrystallization, cytochalasan family |
| 基金项目:中国科学院知识创新工程重要方向项目（No. KSCX2-YW-G-073, KSCX2-EW-G-12B）；广东省科技计划项目（No. 2010B030600010）；广东省科学院优秀青年科技人才基金 |
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| 摘要点击次数: 1987 |
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| 中文摘要: |
| 采用硅胶柱、反相硅胶柱和凝胶柱等色谱技术和重结晶进行分离纯化，从一株分离自南海沉积物的海洋真菌帚状弯孢聚壳Eutypella scoparia的液体发酵提取物中分离得到6个化合物。通过分析波谱数据和与文献比对，化合物1－6分别被鉴定为：(3S,3aR,7aS)-3a,4,5,7a-tetrahydro-3,6-dimethylbenzofuran-2(3H)-one (1)，rel-(3S,6S,7R,10R)-7,10-epoxy-3,7,11- trimethyldodec-1-ene-3,6,11-triol (2)，euphorbol (3)，tuberoside (4)，phenochalasin B (5)，尿嘧啶(6)。生物活性测试结果显示细胞松弛素类化合物5对MCF-7、NCI-H460和SF-268三种肿瘤细胞株均具有显著的抗肿瘤活性，其IC50分别为0.022μmol/L、0.073μmol/L和0.059μmol/L。 |
| 英文摘要: |
| By various chromatographic methods (silica gel, reverse silica gel, Sephadex LH-20 and so on) and recrystallization, six compounds were isolated from the culture of the marine fungus Eutypella scoparia, which was isolated from the sediment of the South China Sea. By extensive NMR analysis as well as comparison with literature reports, the structures of compounds 1?6 were established as (3S,3aR,7aS)-3a,4,5,7a-tetrahydro-3,6-dimethylbenzofuran-2(3H)-one (1), rel-(3S,6S,7R,10R)-7,10-epoxy-3,7,11- trimethyldodec-1-ene-3,6,11-triol (2), euphorbol (3), tuberoside (4), phenochalasin B (5) and uracil (6). Compound 5, which belongs to cytochalasan family, displayed notable cytotoxic activities on MCF-7, NCI-H460 and SF-268 cell growth with IC50 values of 0.022μmol/L, 0.073μmol/L and 0.059μmol/L respectively. |
| 孙利,李冬利,陈玉婵,陶美华,章卫民,但飞君. 海洋真菌帚状弯孢聚壳FS26代谢产物的分离鉴定与抗肿瘤活性研究[J]. 菌物学报, 2011, 30(2): 268-274 |
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