| 一株产石杉碱甲的蛇足石杉内生真菌的分离和鉴定 |
| Producing huperzine A by an endophytic fungus from Huperzia serrata |
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| 中文关键词:蛇足石杉，石杉碱甲，HPLC检测，ITS序列分析，黄曲霉 |
| 英文关键词:Huperzia serrata, huperzine A, HPLC detection, ITS sequence, Aspergillus flavus |
| 基金项目:国家自然科学基金（No. 21066014）；江西省科技厅重大项目（No. 2010BSA19200）；江西省教育厅项目（No. GJJ11602；GJJ11393）；江西师范大学青年成长基金（No. 3189；2708） |
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| 中文摘要: |
| 从药用蕨类植物蛇足石杉叶中分离得到53株内生真菌，在马铃薯葡萄糖液体培养基（PDB）中发酵后对内生真菌发酵提取物液进行TLC、HPLC 和ESI-MS检测，结果表明菌株LF40为产石杉碱甲内生菌株，含量达80.1μg/gdcw，并具有体外乙酰胆碱酯酶抑制活性；运用形态学及ITS-rDNA序列分析方法对菌株LF40进行鉴定，确定为黄曲霉Aspergillus flavus LF40。 |
| 英文摘要: |
| A total of fifty-three endophytic fungal isolates were obtained from fresh leaves of Huperzia serrata, a traditional Chinese medicinal plant. The strain LF40 was found to produce huperzine A (HupA) in potato dextrose broth (PDB) with a yield of 80.1μg /gdcw, based on TLC, HPLC and ESI-MS detection. The inhibition effects of TLC purified fungal HupA from strain LF40 was similar to authentic HupA. Morphological characteristics and internal transcribed spacer (ITS) sequences show that the strain LF40 was Aspergillus flavus LF40. |
| 汪涯,颜日明,曾庆桂,张志斌,汪娣,朱笃. 一株产石杉碱甲的蛇足石杉内生真菌的分离和鉴定[J]. 菌物学报, 2011, 30(2): 255-262 |
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