| 一株产紫杉醇内生真菌液体发酵工艺的优化 |
| A study on optimal fermentation of an endophytic fungus producing taxol |
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| 中文关键词:紫杉醇，南方红豆杉，发酵条件优化，高效液相色谱（HPLC） |
| 英文关键词:taxol, Taxus chinensis, optimal fermentation, high performance liquid chromatography |
| 基金项目:贵州大学创新基金[红豆杉产紫杉醇及其类似物内生真菌的研究（校研农2010006）] |
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| 中文摘要: |
| 紫杉醇是一种高效、低毒、广谱的天然抗癌药物，可以有效地治疗乳腺癌、子宫癌等。近年来的研究发现，从植物内生真菌中发酵生产紫杉醇被证明是解决药源问题的有效途径。从分离到的420株内生真菌中筛选到一株产紫杉醇的内生真菌XC1-07为实验材料进行发酵条件的初步优化。结果表明：最适碳源、氮源分别是麦芽糖和NH4NO3；在含10g/L NH4NO3、90g/L麦芽糖、1.0g/L MgSO4、pH6的优化培养基中培养13d，紫杉醇的产量为1,124.34μg/L。为目前报道的植物内生真菌发酵生产紫杉醇最高的产量。 |
| 英文摘要: |
| Taxol with high efficiency and low toxicity to normal cells and broad-spectrum in inhibiting the growth of tumor cells has been widely used against breast cancer, uterine cancer and other cancers. Recent studies have found that extracting taxol from endophytic fungi has been demonstrated to be a high potential for efficient production of taxol. Taxus was selected as the material for the studies. A total of 420 endophytic fungal was isolated from the roots, stems, leaves, seeds of Taxus at different times and places. The fungus strain XC1-07 isolated from the inner bark of Taxus chinensis was confirmed to produce taxol by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The strain XC1-07 has a taxol yield reaching 780μg/L when grown in potato dextrose liquid medium. The optimal carbon and nitrogen source for mycelium fermentation were maltose and ammonium nitrate respectively. The yield of taxol reached up to 1,124.34μg/L after being incubated in the optimal medium containing 10g/L ammonium nitrate, 90g/L maltose, 1.0g/L magnesium sulfate with pH6. This is the highest yield of taxol production by fermentation of endophytic fungi until now. |
| 靳瑞,康冀川,文庭池,何劲,雷邦星. 一株产紫杉醇内生真菌液体发酵工艺的优化[J]. 菌物学报, 2011, 30(2): 235-241 |
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