| 一种快速高效提取病原真菌DNA作为PCR模板的方法 |
| An efficient extraction method of pathogenic fungus DNA for PCR |
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| 中文关键词:真菌DNA，Chelex-100法，DNA提取 |
| 英文关键词:fungal DNA, Chelex-100 method, DNA extraction |
| 基金项目:高等学校博士学科点专项科研基金（No. 200805650004）；农业部热带作物种质资源专项（农垦办[2009]58） |
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| 中文摘要: |
| 真菌rDNA-ITS序列分析适合于较高等级水平的生物群体间的系统分析。真菌DNA的提取采用传统的方法，步骤繁琐，需要较长时间。采用Chelex-100法提取真菌DNA，使用PCR扩增rDNA-ITS序列评价提取核酸的质量。结果显示，该方法具有经济、简便、快速、高效的特点，是一种比较理想的提取真菌基因组DNA作为PCR模板的方法。 |
| 英文摘要: |
| The fungal rDNA-ITS sequence analysis is suitable for systematic studies of higher level biological populations. However, this traditional method of extraction of fungal genomic DNA is procedurally overelaborate and time-consuming. Chelex-100 was adopted to extract DNA from pathogenic fungi, and the qualities of PCR amplification and rDNA-ITS sequence analysis were evaluated. The results showed that this improved method is simple, high efficient and convenient for pathogenic fungal DNA extraction. |
| 陈吉良,黄小龙,吴安迪,周双清,黄东益,刘进平. 一种快速高效提取病原真菌DNA作为PCR模板的方法[J]. 菌物学报, 2011, 30(1): 147-149 |
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