| 产黄酮成分的绶草内生真菌的鉴定 |
| Endophytic fungi producing flavonoids from Spiranthes sinensis |
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| 中文关键词:绶草，不等弯孢，嗜松青霉 |
| 英文关键词:Spiranthes sinensis, Curvularia inaequalis, Penicillium pinophilum |
| 基金项目:江西省教育厅科技计划项目（No. GJJ09606） |
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| 中文摘要: |
| 利用薄层层析法进行的定性分析表明，分离自绶草Spiranthes sinensis的3株内生真菌产生了黄酮类化合物，在形态学鉴定的同时，扩增、测序了3株内生真菌的rDNA的ITS序列，利用MEGA4.0软件和邻接法（Neighbour-joining methods）进行了聚类分析，菌株S-1鉴定为不等弯孢Curvularia inaequalis，S-2和S-3鉴定为嗜松青霉Penicillium pinophilum。 |
| 英文摘要: |
| Thin-layer chromatography (TLC) analysis indicated that three strains S-1, S-2, and S-3 of endophytic fungi isolated from roots, stems and leaves of Spiranthes sinensis could produce flavonoid. To identify these 3 strains, ITS DNA regions of 3 strains were amplified through PCR reaction and sequenced. The phylogenetic tree was constructed by the software MEGA (version 4.0) using Neighbour-joining methods. Morphological observation and molecular biology identification indicated that the strain S-1 was Curvularia inaequalis., and S-2 and S-3 were Penicillium pinophilum. |
| 刘紫英. 产黄酮成分的绶草内生真菌的鉴定[J]. 菌物学报, 2011, 30(1): 133-137 |
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