| 原生质体再生对糙皮侧耳Pleurotus ostreatus病毒脱除的效果 |
| Effect of protoplast regeneration technology on the virus-elimination of Pleurotus ostreatus |
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| 中文关键词:食用菌，糙皮侧耳，dsRNA技术，脱毒，脱毒菌株，生理性状，糙皮侧耳栽培 |
| 英文关键词:edible fungi, Pleurotus ostreatus, dsRNA technique, virus-elimination, virus-free strains, physiological characteristics, cultivation of Pleurotus ostreatus |
| 基金项目:国家现代农业产业技术体系建设专项资金 |
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| 摘要点击次数: 2010 |
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| 中文摘要: |
| 以糙皮侧耳Pleurotus ostreatus菌株新831和豫6为材料，从这两个菌株的菌丝体中提取了糙皮侧耳病毒基因组，共有3个dsRNA片段，大小分别为8.2kb、2.6kb和1.1kb。采用菌丝尖端分离脱毒、原基组织分离脱毒和原生质体再生脱毒技术对糙皮侧耳菌丝体进行脱毒处理，利用dsRNA技术对脱毒效果进行检测。结果显示，原基组织分离脱毒后3个条带依然存在；菌丝尖端分离脱毒后，8.2kb和1.1kb 2个条带完全脱除，2.6kb的条带亮度有所减弱；原生质体再生脱毒后3个条带完全脱除；对3种脱毒技术得到的菌株进行生理生化指标测定，结果显示，原生质体再生脱毒菌株的菌丝生长速度、生物量、呼吸强度、纤维素酶活等均明显优于出发菌株、原基组织分离脱毒和菌丝尖端分离脱毒菌株；栽培结果表明，原生质体再生脱毒菌株前两茬菇的生物学效率达到96.4%－99.1%，比出发菌株提高19.9%－25.4%，并且菌盖宽度和长度有所增加，表明原生质体再生技术可以有效脱除糙皮侧耳菌株病毒，提高糙皮侧耳栽培产量。 |
| 英文摘要: |
| Three dsRNA bands with sizes of 8.2kb, 2.6kb and 1.1kb respectively, were isolated from two Pleurotus ostreatus strains, Xin-831 and Yu-6. Three virus-eliminated methods, i.e. hyphal tips isolation virus-elimination (HTIVE), primordium tissue isolation virus-elimination (PTIVE) and protoplast regeneration virus-elimination (PRVE) were applied to prepare virus-eliminated strains, and the effect of virus-elimination was detected by dsRNA technique. The results showed that no dsRNA remained in the strains by PRVE method. However, 3 dsRNA bands still remained by PTIVE method and one dsRNA band of 2.6kb, remained by HTIVE method. The results also showed that the hyphal growth rate, biomass, breath intensity and cellulose activity of virus-free strains by PRVE method were obviously superior to the original strains and strains by HTIVE and PTIVE methods. In addition, the biological efficiency of virus-free strains was 96.4% to 99.1%, and 19.9%－25.4% higher than the original strains. Moreover, the length and width of pileus increased. In all, these results indicated that protoplast regeneration technique could effectively eliminate virus of the strains and improve yield of P. ostreatus. |
| 关园园,周素静,王震,麻兵继,高玉千,邱立友,戚元成,申进文. 原生质体再生对糙皮侧耳Pleurotus ostreatus病毒脱除的效果[J]. 菌物学报, 2011, 30(1): 108-115 |
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