| 球囊霉素相关土壤蛋白的提取条件 |
| Extraction conditions of glomalin-related soil protein |
| |
| 中文关键词:丛枝菌根真菌，蛋白提取，离心力，灭菌，土样质量 |
| 英文关键词:arbuscular mycorrhizal fungi, extraction of protein, centrifugal force, sterilization, soil sample mass |
| 基金项目:国家自然科学基金（No. 30870458，31070103）；广东省自然科学基金（No. 10251007002000001） |
|
| 摘要点击次数: 1800 |
| 全文下载次数: 1356 |
| 中文摘要: |
| 球囊霉素是由丛枝菌根真菌分泌的一种含金属离子的糖蛋白，因被非专一性提取而称为球囊霉素相关土壤蛋白（GRSP）。由于提取条件能影响GRSP的测定值，因此研究了柑橘园土样前期灭菌、离心力和土样质量等3个提取条件对GRSP测定值的影响。结果表明，土样前期灭菌和较高的离心力（15,000′g）均能显著影响GRSP的测定值；在土样质量0.25－1.0g范围内，GRSP的测定值随着土样质量的提高而升高；经过3次和6次的连续提取，总球囊霉素相关土壤蛋白的累积量分别达到理论最大值的66.8%和92.1%以上。研究结果为GRSP测定的标准化提供了依据。 |
| 英文摘要: |
| Glomalin is a soil glycoprotein produced by arbuscular mycorrhizal (AM) fungi, presently named as glomalin-related soil protein (GRSP) due to its unspecific extraction. The extraction conditions of GRSP can affect its measured value, therefore, the influences of pre-sterilization of soil sample, centrifugation and soil sample mass on the measured value of GRSP were studied with citrus orchard soil. The results showed that both pre-sterilization of soil sample and centrifugation at higher centrifugal force (15,000′g) significantly affected the measured value of GRSP, indicating in the range of 0.25－1.0g the greater of soil sample mass, the higher of the measured value showed. The cumulative contents of total GRSP exceeded 66.8% and 92.1% of the theoretical maximum, respectively. These results provide a basis for the standardization of GRSP measurement, and also contribute to the comparison among the measured values of GRSP in literatures. |
| 谢小林,许朋阳,朱红惠,姚青. 球囊霉素相关土壤蛋白的提取条件[J]. 菌物学报, 2011, 30(1): 92-99 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|