| 金沙江干热河谷地区芒果畸形病的病原菌 |
| The pathogen causing mango malformation in Jinsha River Dry-hot Valley, China |
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| 中文关键词:镰刀菌，形态学，EF-1α |
| 英文关键词:Fusarium mangiferae, morphology, EF-1α |
| 基金项目:科技部农业科技成果转化资金项目（No. 2008GB23260386）；公益性行业（农业）科研专项（No. nyhyzx07-032-3）；中央级非盈利性研究机构基本科研业务费研究专项（No. sscri201002） |
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| 中文摘要: |
| 从金沙江干热河谷地区采集芒果畸形病组织，运用柯赫氏法则证实分离物MG6为该病的致病菌。菌株MG6在马铃薯蔗糖琼脂培养基（PSA）上菌丝白色，无色素产生，米饭培养基浅粉红色；在康乃馨叶片培养基（CLA）上以单瓶梗或复瓶梗假头状产孢，不产生链状孢子；小型分生孢子卵形或长椭圆形，具0－1个分隔，3.1－10.2×1.5－2.2μm；大型分生孢子呈镰刀形，通常3个分隔，18－38×1.8－2.4μm。EF-1α测序结果在Fusarium数据库中进行同源性分析显示，菌株MG6与F. mangiferae的同源性最高，达99.68%。综合培养性状、形态学特征和EF-1α序列分析，将菌株MG6鉴定为Fusarium mangiferae。 |
| 英文摘要: |
| Mango tissue samples infected by malformation disease were collected from Jinsha River Dry-hot Valley and the fungal strains were isolated after surface sterilization. Isolate MG6 was confirmed to be the pathogen of this disease through Koch’s rules. Based on morphological characteristics and EF-1α sequencing, isolate MG6 was identified as Fusarium mangiferae. The colony of the strain was white and pigment was not formed on Potato Sugar Agar (PSA). Pigmentation on rice medium was pink. On Carnation Leaf Agar (CLA) medium, microconidia formed false head on branched monophialides or polyphialides, and conidia in chains were not observed. Microconidia are ovate to long-oval, 0－1-septate, 3.1－10.2×1.5－2.2μm. Macroconidia are falculate, 3－5-septate, 18－38×1.8－2.4μm. Chlamydospores are absent. The EF-1α region of the pathogen was amplified and sequenced. A blast search through the Fusarium database (http://isolate.fusariumdb.org) showed that the identity of EF-1α sequence of isolate MG6 to that of Fusarium mangiferae NRRL 25226, the closest match was 99.68%, confirming the morphological identification of the isolate. |
| 杨顺锦,詹儒林,柳凤,赵艳龙,常金梅,何衍彪. 金沙江干热河谷地区芒果畸形病的病原菌[J]. 菌物学报, 2011, 30(1): 27-31 |
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