青檀内生真菌菌群多样性的研究
Diversity of endophytic fungi from Pteroceltis tatarinowii
  
中文关键词:青檀,分离率,优势菌群,多样性指数,相似性系数
英文关键词:Pteroceltis tatarinowii, isolation rate, dominant population, diversity index, similarity coefficient
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作者单位
柴新义 南京师范大学生命科学学院 江苏省生物多样性与生物技术重点实验室 江苏省微生物资源产业化工程技术研究中心 南京 210046 滁州学院化学与生命科学系 滁州 239012 
陈双林 南京师范大学生命科学学院 江苏省生物多样性与生物技术重点实验室 江苏省微生物资源产业化工程技术研究中心 南京 210046 
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中文摘要:
      为了解我国特有孑遗植物青檀的内生真菌的菌群组成和变化规律,分别于春秋两季从江苏、安徽、河南和山东4省的5个野生青檀居群地采样进行了内生真菌的分离、鉴定及多样性分析。结果表明,所分离出的3,611株内生真菌归于31属,无性型子囊菌中的腔孢类菌群占49.42%、丝孢类菌群占28.52%,有性型子囊菌菌群占2.52%,其余19.52%的菌群不产孢。在真菌属的水平,以拟茎点霉属Phomopsis(22.10%)和交链孢属Alternaria(20.66%)为优势菌群。南京与枣庄的野生青檀内生真菌菌群组成之间的相似性最高(Cs=0.84),南京与南阳的相似性最低(Cs=0.60)。在宿主组织中,青檀叶片、叶柄和枝条中都以腔孢类菌群居于优势。春季和秋季青檀内生真菌的组成存在较大差异,春季以丝孢类菌群居多(46.71%),秋季则以腔孢类菌群居多(55.85%)。
英文摘要:
      Diversity of endophytic fungi from Pteroceltis tatarinowii was studied in order to understand diversity and succession change of endophytic fungal communities in this endemic plant. Healthy plant samples were collected from five natural distribution areas of P. tatarinowii in Anhui, Henan, Shandong and Jiangsu Provinces. A total of 3,611 isolates of endophytic fungi were obtained. Thirty-one genera belonging to Ascomycota (2.25%), Coelomycetes (49.42%) and Hyphomycetes (28.52%) were identified, of these Phomopsis (22.10%) and Alternaria (20.66%) were the dominant genera. A total of 19.52% of all isolates were sterile on the media. The community of endophytic fungi from P. tatarinowii of Nanjing and that of Zaozhuang has the highest similarity (Cs = 0.84), while the community similarity of Nanjing and that of Nanyang was the lowest (Cs = 0.60). Coelomycetes was the most dominant in lamina, petiole and branch. The isolation rate of Hypomycetes was the highest (46.71%) in spring, but that of Coelomycetes (55.85%) was the highest in autumn.
柴新义,陈双林. 青檀内生真菌菌群多样性的研究[J]. 菌物学报, 2011, 30(1): 18-26
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