| 猴头菌水溶性多糖的分离纯化与结构表征 |
| Isolation and structural characterization of a water-soluble polysaccharide from Hericium erinaceus |
| |
| 中文关键词:大型真菌，传统中草药，杂多糖，纯化，结构分析 |
| 英文关键词:fungi, Chinese traditional herb, heteropolysaccharide, purification, structure elucidation |
| 基金项目:Leading Academic Discipline Project of Shanghai Municipal Education Commission (No. J50704) |
|
| 摘要点击次数: 2035 |
| 全文下载次数: 1068 |
| 中文摘要: |
| 从猴头菌子实体中分离得到一种新型的水溶性杂多糖HEPF2，分子量大小为1.66′104Da，该多糖由岩藻糖、半乳糖和葡萄糖以1.00:3.69:5.42比例构成，同时也含有微量的3-O-甲基鼠李糖。进一步利用傅立叶变换红外光谱法、糖组成分析、甲基化分析、部分酸水解法和核磁共振法等方法进行结构鉴定，检测结果表明，该杂多糖中包含1→4、1→6结合的葡萄糖和1→6结合的半乳糖残基，连接于主链的侧链残基，包括岩藻糖残基、少数的端基葡萄糖和半乳糖残基。核磁共振法检测结果还表明，1→4结合葡萄糖为β构型，(1→6)结合半乳糖、(1→2,6)结合半乳糖和端基葡萄糖均为α构型。 |
| 英文摘要: |
| A novel water-soluble heteropolysaccharide termed HEPF2 was isolated from the fruiting bodies of Hericium erinaceus. HEPF2 has a molecular weight of 1.66′104Da and is composed of fucose, galactose and glucose at a ratio of 1.00:3.69:5.42. HEPF2 also contains a small amount of 3-O-methylrhamnose. Its structural characteristics were further investigated by using combined technologies including FT-IR, sugar and methylation analysis, partial acid hydrolysis and NMR spectroscopy. The results showed that HEPF2 was a heteropolysaccharide chemically featured by (1→4)-linked glucosyl, (1→6)-linked glucosyl and (1→6)-linked galactosyl residue, attached to the O-2 of main chain with terminal fucosyl residue or a minor of terminal glucosyl and galactosyl residues. NMR spectra showed that (1→4)-linked glucosyl was β configuration, while (1→6)-linked galactosyl, (1→2,6)-linked galactosyl together with the terminal fucosyl residue were all α configuration. |
| 张安强,傅立,孙培龙,张劲松,潘迎捷. 猴头菌水溶性多糖的分离纯化与结构表征[J]. 菌物学报, 2010, 29(6): 911-917 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|