| 一株产白藜芦醇虎杖内生真菌的分离和鉴定 |
| Isolation and identification of an endophytic fungus producing resveratrol in Polygonum cuspidatum |
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| 中文关键词:药用植物，HPLC检测，ITS序列分析，拟轮枝镰孢菌 |
| 英文关键词:medicinal plant, HPLC detection, ITS sequence, Fusarium verticillioides |
| 基金项目:陕西省自然科学基金（No. 2003C1-21）；陕西省教育厅科研基金（No. 04JK159） |
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| 中文摘要: |
| 从药食同源植物虎杖根中分离得到15株内生真菌，在马铃薯葡萄糖液体培养基（PDB）中发酵后对所有内生真菌发酵液进行HPLC检测，有5株菌的发酵液中相关成分与白藜芦醇标准品保留时间一致，确定5株菌能产生白藜芦醇，在此基础上测定了5株菌的生长曲线。其中菌株H8生长最旺盛，白藜芦醇产量最高。应用形态学及ITS序列分析方法对其进行鉴定，确定为拟轮枝镰孢菌Fusarium verticillioides。 |
| 英文摘要: |
| The fresh roots of Polygonum cuspidatum, a traditional Chinese medicine plant, were used to isolate endophytic fungi. Fifteen strains were obtained. Five isolates producing resveratrol were investigated based on HPLC detection after fermentation in potato dextrose broth (PDB). Strain H8 was the most prospective in biological production of resveratrol. Morphological characteristics and internal transcribed spacer (ITS) sequences show that strain H8 was Fusarium verticillioides. |
| 刘芸,殷红,仇农学. 一株产白藜芦醇虎杖内生真菌的分离和鉴定[J]. 菌物学报, 2010, 29(4): 502-507 |
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