| 煤附生真菌产漆酶菌株的分离鉴定及产酶特性研究 |
| Identification and production of laccase-producing fungal strain isolated from coal |
| |
| 中文关键词:均匀设计，半知菌，棘孢木霉，ITS序列 |
| 英文关键词:uniform design, deuteromycetes, Trichoderma asperellum, ITS sequence |
| 基金项目:重庆市科委科技攻关资助项目（No. CSTC, 2006AC1045） |
|
| 摘要点击次数: 1557 |
| 全文下载次数: 837 |
| 中文摘要: |
| 从煤炭样品中筛选到一株产漆酶活性菌株，经菌体形态观察和ITS序列分析，鉴定为Trichoderma asperellum W03。菌株所产漆酶的最适反应pH为3.5－4.5，最适反应温度45℃，类似于白腐真菌漆酶。液态发酵条件的均匀设计实验表明，适宜的发酵培养基组成为：土豆200.00g/L、葡萄糖9.36g/L、米糠粉37.44g/L、硝酸钾 4.00g/L、KH2PO4 3.20g/L、MgSO4?7H2O 2.00g/L、CuSO4?5H2O 0.005g/L、初始pH8.0；在33℃、180r/min、50mL/250mL的摇瓶培养条件下，棘孢木霉W03在孢子接种培养后48h、84h产酶量较高，分别处在菌体的快速生长期和衰亡期；菌体产酶受Cu2+、联苯胺诱导，而受1-萘酚、愈创木酚和2,4-D抑制。 |
| 英文摘要: |
| Laccase-producing strain screened from low-rank coal was identified as Trichoderma asperellum W03 according to its mycelia morphology and ITS sequence analysis. The laccase has an optimum pH of 3.5－4.5 and an optimum temperature of 45℃, which is similar to that from white rot fungi. Components of laccase-producing medium (g/L) optimized by uniform design were 200.00g/L potato, 9.36g/L glucose, 37.44g/L rice bran powder, 4.00g/L KNO3, 3.20g/L KH2PO4, 2.00g/L MgSO4?7H2O and 0.005g/L CuSO4?5H2O. The peak of laccase production appeared in the 48h and 84h corresponding to the logarithmic phase and decline phase, respectively, when Trichoderma asperellum W03 was submerged in shaking-flask with liquid volume 50mL/250mL under constant temperature of 33℃ and vibration of 180r/min. The laccase production in Trichoderma asperellum W03 was significantly improved by addition of some substance such as potato extract, rice bran, Cu2+ or benzidine in the medium, but obviously inhibited by 1-naphthol, guaiacol and 2,4-D. In addition, the suitable initial pH for laccase production was 8.0. |
| 陈今朝,王剑锋,李江,饶军,秦家顺. 煤附生真菌产漆酶菌株的分离鉴定及产酶特性研究[J]. 菌物学报, 2010, 29(3): 389-396 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|