| 毛头鬼伞子实体中甾类化合物的结构鉴定及其抑制肿瘤细胞增殖活性的研究 |
| Steroids from fruiting bodies of Coprinus comatus and their inhibition to tumor cell proliferation |
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| 中文关键词:食药用真菌，次生代谢产物，核磁共振，细胞毒性 |
| 英文关键词:edible and medicinal fungi, secondary metabolites, nuclear magnetic resonance, cytotoxicity |
| 基金项目:上海市农委重点攻关项目（No. 农科攻字2003-3-3） |
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| 中文摘要: |
| 从毛头鬼伞子实体中分离得到4个甾类化合物，通过波谱分析，分别鉴定为麦角甾醇（1）、啤酒甾醇（2）、麦角甾醇葡萄糖甙（3）和tuberoside（4）。4个化合物均为首次从毛头鬼伞中得到。通过体外细胞毒性筛选试验，结果表明化合物4有较强的抑制人乳腺癌细胞MCF-7和狗肾细胞MDCK增殖的活性，其抑制增殖的IC50值分别为10.9μg/mL（18.4μmol/L）和5.8μg/mL（9.8μmol/L）。化合物3对MCF-7和MDCK的抑制作用则较弱，当其浓度为10.0μg/mL（17.9μmol/L）时，对MCF-7和MDCK的增殖抑制率分别为12.5%和7.5%。 |
| 英文摘要: |
| Four steroids were isolated from fruiting bodies of Coprinus comatus by using column chromatography. On the basis of spectral data, they were identified as ergosterol (1), cerevisterol (2), ergosteryl-3-O-β-D-glucopyranoside (3), and tuberoside (4). All these compounds were isolated from this fungus for the first time. By in vitro cytotoxicity screening, compounds 3 and 4 were found to inhibit the proliferation of MCF-7 tumor cells and MDCK cells. Compound 4 showed strong inhibition to the proliferations of MCF-7 and MDCK at IC50 of 10.9μg/mL (18.4μmol/L) and 5.8μg/mL (9.8μmol/L) respectively. Compound 3 showed weak inhibition to MCF-7 and MDCK’s proliferations with inhibition rates of 12.5% and 7.5% respectively at 10.0μg/mL (17.9μmol/L). |
| 冯娜 张劲松 唐庆九 郝瑞霞 刘艳芳 杨焱 贾薇
周帅 唐传红 钟建江. 毛头鬼伞子实体中甾类化合物的结构鉴定及其抑制肿瘤细胞增殖活性的研究[J]. 菌物学报, 2010, 29(2): 249-253 |
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