| 肌动蛋白是扁绒泡菌Physarum compressum原质团细胞核核骨架成分 |
| Actin is one of the constituents of nuclear skeleton in plasmodia of Physarum compressum |
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| 中文关键词:黏菌，核基质，免疫印迹 |
| 英文关键词:myxomycetes, nuclear matrix, Western blot |
| 基金项目:国家自然科学基金（No. 30770005）；吉林农业大学博士启动基金（No. 203136） |
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| 中文摘要: |
| 本研究以2mol/L NaCl或3, 5-二碘水杨酸锂（Lis）抽提扁绒泡菌Physarum compressum原质团细胞核，对残余蛋白进行电泳及肌动蛋白免疫印迹分析。结果表明单独用Lis抽提细胞核不能有效去除组蛋白，用2mol/L NaCl则可有效去除组蛋白，免疫印迹实验表明核骨架中存在着可以有效对抗2mol/L NaCl的肌动蛋白。 |
| 英文摘要: |
| Nuclear matrix residual protein was obtained by digesting and extracting Physarum compressum phaneroplasmodium nuclei, using 2mol/L NaCl and 3, 5-diiodosalicylic acid lithium salt (Lis) respectively. SDS-PAGE analysis of residual matrix protein and Western blot analysis of actin indicated that the histons can’t be removed by using Lis alone but can be removed by using 2mol/L NaCl. Actin in the nuclear matrix was resistant to 2mol/L NaCl effectively. |
| 王晓丽 李艳双 盖玉红 李玉. 肌动蛋白是扁绒泡菌Physarum compressum原质团细胞核核骨架成分[J]. 菌物学报, 2010, 29(2): 185-187 |
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