一种狗尾草病原真菌的鉴定及菌株致病性研究
Identification and pathogenicity of fungus on Setaria viridis
  
中文关键词:狗尾草平脐蠕孢,致病性,ITS序列
英文关键词:Bipolaris setariae, pathogenicity, ITS sequence
基金项目:国家自然科学基金(No. 30600010)
作者单位
赵杏利 邓晖 牛永春 中国农业科学院农业资源与农业区划研究所 北京 100081 
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中文摘要:
      经形态学鉴定和rDNA ITS序列分析,16株分离自北京、河北、河南发病狗尾草的菌株、2株分别分离自河南发病虎尾草、牛筋草的菌株和1株分离自青海发病野燕麦的菌株被鉴定为狗尾草平脐蠕孢Bipolaris setariae。接种试验表明,来自狗尾草的菌株比来自其他寄主植物的菌株对狗尾草致病性强,分离自野燕麦的菌株对狗尾草无致病性,分离自不同地区不同样品狗尾草的菌株其致病性有显著差异。菌株NY1对狗尾草有很强致病性,接种后5d植株叶片即全部呈枯死状,接种后7d整个植株枯萎死亡。菌株NY1对马唐和虎尾草也有很强致病性,但对于大多数供试栽培植物致病性很弱或无致病性。因此,B. setariae NY1菌株具有进一步开发成为狗尾草、马唐和虎尾草等杂草的生物除草剂的潜力。
英文摘要:
      Based on morphological characteristics and sequence alignment analysis of rDNA ITS region, 16 strains isolated from diseased plants of Setaria viridis from Beijing, Hebei and Henan, two strains isolated from diseased plants of Chloris virgata and Eleusine indica from Henan, and one strain isolated from diseased plant of Avena fatua from Qinghai, were identified as Bipolaris setariae. Artificial inoculation tests indicated that the strains from S. viridis had stronger pathogenicity than those from other hosts to S. viridis. The strain from A. fatua had no pathogenicity to S. viridis. The strains with different origins showed difference in virulence to S. viridis. The strain NY1 was strongly pathogenic to S. viridis. All the leaves blighted in 5 days after inoculation, and the whole plants died in 7 days. NY1 was also strongly pathogenic to the weeds C. virgata and Digitaria sanguinalis but had light or no pathogenicity to most crops. The B. setariae NY1 strain is potential to be developed as a microbial herbicide to the weeds S. viridis, C. virgata and D. sanguinalis.
赵杏利 邓晖 牛永春. 一种狗尾草病原真菌的鉴定及菌株致病性研究[J]. 菌物学报, 2010, 29(2): 172-177
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