| α-鹅膏毒肽（α-AMA）的制备及其对肝癌细胞SMMC-7721和H22荷瘤小鼠的抑瘤作用研究 |
| Purification and inhibiting effects on SMMC-7721 cell and mice-bearing H22 tumor of α-amanitin (α-AMA) |
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| 中文关键词:树木溃疡病，Botryosphaeria dothidea，B. berengeriana，B. ribis，MP系统进化树 |
| 英文关键词:tree canker disease, Botryosphaeria dothidea, B. berengerian, B. ribis, MP phylogeny tree |
| 基金项目:吉林省教育厅科技计划项目 |
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| 摘要点击次数: 1932 |
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| 中文摘要: |
| 对收集的22株和GenBank下载的40株葡萄座腔菌Botryosphaeria ITS序列构建了MP（Maximum parsimony）进化树。在以Leucostoma为外群的情况下，来自我国（主要是陕西）的葡萄座腔菌树木溃疡病菌总体上可分为2个大群，BDGroup1 与GenBank上报道的B. dothidea 被聚在一起，BD Group2菌株全部来自我国关中咸阳，独立成群，与Botryosphaeria属其他种亲缘关系远。培养特征、分生孢子大小和致病性测定显示：来自我国的苹果轮纹病菌、苹果干腐病菌、梨轮纹病菌、桃树流胶病菌同B. dothidea亲缘关系近，在MP树中聚在一起（BD Group1）。ITS序列分析结果支持B. dothidea与B. berengeriana为同物异名的观点。 B. ribis 与B. parva亲缘关系近，而与B. dothidea亲缘关系远，支持B. ribis与B. dothidea为不同种的观点。 |
| 英文摘要: |
| Based on maximum parsimony phylogeny tree of ITS-nrDNA sequences of 22 isolates collected from Shaanxi Province and 40 isolates downloaded from GenBank, Botryosphaeria species from China were clustered into two groups: BDGroup1 was composed of B. dothidea isolates, and BDGroup2 from Guanzhong region of Shaanxi Province was a distant related group. Cultivation characteristics, conidial size and pathogenicity of the Chinese isolates indicated that pathogens of apple ring rot disease, apple stem dry rot disease, pear ring rot disease and peach gummosis had high homogenous with B. dothidea, and they were all clustered in BDGroup1, supporting the idea that B. dothide and B. berengeriana are synonymous. The MP (Maximum parsimony) tree also showed that B. ribis was not the synonym species of B. dothidea, and B. ribis was more homogenous with B. parva Pennycook & Samuels. The other Botryosphaeria species were also discussed in this paper. |
| 徐璐,包海鹰. α-鹅膏毒肽（α-AMA）的制备及其对肝癌细胞SMMC-7721和H22荷瘤小鼠的抑瘤作用研究[J]. 菌物学报, 2010, 29(1): 138-142 |
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