| 紫芝酸性三萜类化合物体外抑癌和抑菌作用的研究 |
| In vitro anti-tumor and antimicrobial activity of acidic triterpenoids from submerged culture of Ganoderma sinense |
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| 中文关键词:胞内酸性三萜，胞外酸性三萜，抗癌作用，抑菌活性 |
| 英文关键词:intracellular acidic triterpenoids, extracellular acidic triterpenoids, anticancer action, antimicrobial activity |
| 基金项目:国家自然科学基金（No. 30700552）；湖南省教育厅科研项目（No. 07C796） |
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| 中文摘要: |
| 采用噻唑蓝比色（MTT）法研究紫芝酸性三萜对几种癌细胞体外增殖的影响，并用管碟法检测了紫芝胞内酸性三萜对几种细菌和霉菌的体外抑菌作用。结果表明，紫芝胞内酸性三萜和胞外酸性三萜在250μg/mL时，对人肝癌细胞BEL7402和人乳腺癌细胞MCF-7均有显著抑制作用（P < 0.05），但对人胃癌细胞SGC-7901没有显著抑制作用（P > 0.05）。BEL7402细胞的生长曲线试验表明，胞内酸性三萜组的细胞受到显著抑制，未出现指数增长期，且BEL7402细胞培养3d后，对照组细胞数目多、均匀，而胞内酸性三萜组的细胞数目明显减少，且细胞变小。抑菌试验结果表明，胞内酸性三萜在40mg/mL时，对大肠杆菌Escherichia coli和金黄色葡萄球菌Staphylococcus aureus的生长均具有显著抑制作用（P < 0.01），对枯草芽孢杆菌Bacillus subtitis和青霉的Penicillium chrysogenum抑制作用较弱；而在此浓度下对黑曲霉Aspergillus niger没有抑制作用。该样品对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、黑曲霉和青霉的MIC分别为20mg/mL、20mg/mL、40mg/mL、80mg/mL和40mg/mL。此外，该酸性三萜的抑菌成分在60℃下（处理2h）较稳定，但在80℃以上，热稳定性较差，活性降低。 |
| 英文摘要: |
| The in vitro anti-tumor activity of acidic triterpenoids from Ganoderma sinense mycelia and submerged fermentation liquid by using 3-(4, 5-dimethylthiozol -2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method, as well as the antimicrobial activity on several bacteria and molds by using cylinder-plate method were investigated. The results showed that both the intracellular and extracellular acidic triterpenoids at 250μg/mL displayed significant inhibitory effects on human hepatoma cell line BEL7402 and human breast carcinoma cell line MCF-7 (P < 0.05); however, they had no significant inhibitory effects on human gastric cancer cell Line SGC-7901. The growth of BEL7402 cells treated by intracellular acidic triterpenoids was significantly inhibited, and there was no exponential phase in the growth curve. After 3d culture, BEL7402 cells treated by intracellular acidic triterpenoids appeared fewer and smaller as compared with the cells in control. The acidic triterpenoids at 40mg/mL had significantly inhibitory effect on the growth of Escherichia coli and Staphylococcus aureus (P < 0.01), however, the sample had little inhibitory effect on Bacillus subtitis and Penicillium chrysogenum, and had no inhibitory effect on Aspergillus niger. Further studies revealed that the minimal inhibitory concentration (MIC) of the acidic triterpenoids to E. coli, S. aureus, B. subtitis, A. niger and P. chrysogenum was 20mg/mL, 20mg/mL, 40mg/mL, 80mg/mL and 40mg/mL, respectively. The antibacterial components of the acidic triterpenoids sample couldn’t be destroyed in 60℃ treatment for 2h, but it could be partly destroyed in 80℃ treatment for 2h, thus the antimicrobial activity was decreased. |
| 王晓玲 刘高强 周国英 赵艳 李杜. 紫芝酸性三萜类化合物体外抑癌和抑菌作用的研究[J]. 菌物学报, 2009, 28(6): 838-845 |
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