| 斜生褐孔菌多糖诱导人肝癌HepG-2细胞凋亡的研究 |
| Proliferation inhibition and apoptosis induction of human hepato-carcinoma HepG-2 cells by Phaeoporus obliquus polysaccharides |
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| 中文关键词:大型真菌，多糖，抗肿瘤，Bcl-2基因，Bax基因 |
| 英文关键词:macrofungi, polysaccharides, antitumor, Bcl-2 gene, Bax gene |
| 基金项目:国家科技支撑项目（No. 2008BADA1B01） |
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| 摘要点击次数: 2011 |
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| 中文摘要: |
| 研究了斜生褐孔菌多糖对人类肝癌HepG-2细胞凋亡的诱导作用。采用噻唑蓝法（MTT法）观察了斜生褐孔菌多糖对HepG-2细胞生长的影响，用透射电镜观察了细胞形态，用DNA Ladder检测了细胞凋亡，用流式细胞仪检测了细胞凋亡率；同时采用逆转录-聚合酶链反应法（RT-PCR法）研究了不同浓度斜生褐孔菌多糖作用后HepG-2细胞中Bax和Bcl-2基因mRNA转录水平的变化。结果表明斜生褐孔菌多糖能抑制HepG-2细胞增殖，并呈时间剂量依赖关系；电镜观察、DNA Ladder和流式细胞仪检测均证实了斜生褐孔菌多糖能够诱导HepG-2细胞凋亡；经斜生褐孔菌多糖处理后，HepG-2细胞中Bax基因 mRNA转录水平增强，而Bcl-2 基因mRNA无明显变化。证明了斜生褐孔菌多糖具有抑制HepG-2细胞生长及诱导HepG-2细胞凋亡的作用，这可能与调节Bcl-2和Bax基因表达水平有关。 |
| 英文摘要: |
| The effects of Phaeoporus obliquus polysaccharides on apoptosis of hepato-carcinoma HepG-2 cells were investigated. The inhibition of cell growth was determined by mono-nuclear cell direct cytotoxicity assay (MTT); morphological changes were observed by transmission electron microscopy; apoptosis was assessed by DNA ladder and flow cytometry (FCM). The mRNA expression levels of Bcl-2 and Bax genes were detected by reverse transcription-polymerase chain reaction (RT-PCR). Phaeoporus obliquus polysaccharides inhibited the growth of HepG-2 cells in vitro in dose-and time-dependent manner. Microscopic observation showed that the cells presented some morphologic features of apoptosis. Agarose gel electrophoresis analysis showed DNA ladder; hypodiploid peak appeared on flow cytometry (FCM) histogram, and the apoptotic rate of HepG-2 cells was risen with the increase of dose. The Bax mRNA expression was remarkably up-regulated in HepG-2 cells exposed to Phaeoporus obliquus polysaccharides, but no apparent changes of Bcl-2 gene were observed. Phaeoporus obliquus polysaccharides inhibit proliferation of HepG-2 cells and induce apoptosis. This may associate with upregulating of the expression of Bax gene. |
| 安晓丽 赵敏 董露璐 何平平 相文华. 斜生褐孔菌多糖诱导人肝癌HepG-2细胞凋亡的研究[J]. 菌物学报, 2009, 28(6): 819-824 |
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