| 正己醇降解菌的分离、筛选及分类鉴定 |
| Screening and identification of hexanol- degrading fungi |
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| 中文关键词:生物降解，地霉属，白地霉，分离筛选，分类鉴定 |
| 英文关键词:bio-degradation, Geotrichum, Geotrichum candidum, isolation, DNA sequence analysis |
| 基金项目:国家自然科学基金项目（No. 20862014）；现代葡萄产业技术体系建设专项（No. z225020804）；陕西省科技攻关项目（No. 2008K02-09） |
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| 中文摘要: |
| 为获得可降解正己醇的真菌菌种，分别以苹果园土壤、苹果渣、苹果酒醪和醋醅为分离源，采用富集培养和紫外线定向诱变，得到了两株能在pH3.8-4.0的条件下降解正己醇的真菌菌株TF和TM。菌株TM和TF在马铃薯葡萄糖培养液中对4.0mg/mL正己醇的降解率分别为45.60±5.43%和23.82±9.27%，与对照在α=0.01水平上差异显著。结合形态学特征及26S rDNA D1/D2区（菌株TM）和ITS区（菌株TF）序列分析，对两株菌进行了分类鉴定。结果表明：菌株TM属于地霉属 Geotrichum，菌株TF为白地霉Geotrichum candidum（有性型Galactomyces geotrichum）。 |
| 英文摘要: |
| In order to obtain the hexanol-degrading fungi, the methods of culture enrichment and UV induction were used in this study. To isolate useful fungi, different materials including apple orchard soil, apple pomace, apple cider mash and vinegar dregs were tested and two valuable fungal strains of TF and TM were obtained from apple orchard soil. The strains showed high ability in degrading hexanol at pH3.8－4.0. During growth in potato dextrose broth (PDB) containing 4.0mg/mL hexanol for 7 days, TM and TF degraded hexanol by 45.60±5.43% and 23.82±9.27%, respectively, significantly different from the control. Based on the morphological characteristics and sequence analysis of 26S rDNA D1/D2 for strain TF and nrDNA ITS for strain TM, TM was identified as Geotrichum sp. and TF as Geotrichum candidum (telemorph, Galactomyces geotrichum). |
| 令桢民 师俊玲 杨保伟. 正己醇降解菌的分离、筛选及分类鉴定[J]. 菌物学报, 2009, 28(6): 769-775 |
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