| 金龟子绿僵菌附着胞分化及其与环腺苷酸cAMP的关联性研究 |
| Appressorial differentiation and its association with cAMP in the insect pathogenic fungus Metarhizium anisopliae |
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| 中文关键词:昆虫病原真菌，体壁穿透，粘液层，类枯草杆菌蛋白酶 |
| 英文关键词:Insect pathogenic fungus, cuticle penetration, mucilaginous sheath, subtilisin |
| 基金项目:The Knowledge Innovation Program of CAS (No. KSCX2-YW-G-037) and the Science and Technology Commission of Shanghai Municipality (No. 07PJ14101) |
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| 中文摘要: |
| 寄主识别与附着胞分化是虫生真菌启动侵染过程的首要步骤。本文利用先前获得的金龟子绿僵菌基因缺失突变株与其野生型一起进行附着胞分化研究。接种后不同时间下的观察表明，绿僵菌突变株或野生型的附着胞既可以在萌发不久的芽管顶端形成，也可以在伸长菌丝分支的顶端形成。与野生型不同的是，突变株附着胞的分化频率显著下降，附着胞周围也缺乏粘液层的产生。研究表明，绿僵菌的类枯草杆菌类体壁降解酶对于附着胞分化不产生影响，对体壁降解也非完全必需的。与突变株附着胞分化频率显著降低相对应，其胞内环腺苷酸cAMP水平显著下降，而添加外源cAMP能够显著增加其附着胞分化频率，说明绿僵菌cAMP信号途径对于调控附着胞分化起着重要的作用。 |
| 英文摘要: |
| Host recognition and appressorium differentiation are the pivotal steps for insect pathogenic fungi to initiate infection process. In this study, appressorial differentiation was studied by comparing a mutant, which has been identified with the loss of genetic materials, with the wild-type strain of the insect pathogenic fungus Metarhizium anisopliae. The observations showed that both the mutant and wild-type strain could not only produce appressoria on the tips of newly germinated spores, but also form multiple infection structures from the terminals of branching mycelia on insect cuticle. In contrast to the wild-type, the frequency of appressorial formation was significantly reduced and in addition, no clear mucilaginous sheath was produced by the mutant appressoria. The study shows that the cuticle degrading enzyme subtilisin is not involved in appressorial differentiation, or indispensable in cuticle digestion. A significantly low frequency of appressorial differentiation by the mutant was coincided with its lower intracellular cAMP level in comparison to the wild-type. Addition of exogenous cAMP could significantly increase the frequency of appressorial differentiation by the mutant, indicating that cAMP signaling pathway is potentially involved in regulation of appressorial differentiation in M. anisopliae. |
| 段志兵 高强 吕丁丁 石少华 BUTT Tariq M. 王成树. 金龟子绿僵菌附着胞分化及其与环腺苷酸cAMP的关联性研究[J]. 菌物学报, 2009, 28(5): 712-717 |
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