| 斑玉蕈低温胁迫下菌丝体酶活变化及差异蛋白质组学研究 |
| Differential proteomics and enzyme changes of Hypsizigus marmoreus mycelium under cold stress |
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| 中文关键词:低温调控，差异蛋白，保护酶 |
| 英文关键词:low temperature control, differentially expressed protein, protected enzyme |
| 基金项目:福建省发改委农业“五新”推广项目[No. (2008)1119] |
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| 中文摘要: |
| 采用蛋白质组学的方法和技术，通过比较最适温度和低温胁迫条件下斑玉蕈菌丝体蛋白质组的变化，寻找与低温胁迫紧密相关的差异蛋白，图谱分析有26个差异表达的蛋白，MALDI-TOF-MS/MS鉴定和数据库检索有14个蛋白得到初步鉴定，其中肌动蛋白、微管蛋白是细胞骨架的重要成分；蛋白酶体和乙酰乳酸合酶参与蛋白质的合成与分解；ATP合成酶、转醛醇酶、甲酸脱氢酶、1,5-二磷酸羧化酶参与细胞内的物质与能量代谢；鸟苷酸结合蛋白、14-3-3蛋白参与信号转导调控；新生多肽相关复合物可能参与细胞分化、发育的调控。说明斑玉蕈在低温胁迫下的调控可能是一个比较复杂的多蛋白和酶参与的过程。通过酶活测定发现，低温胁迫下过氧化氢酶（CAT）、过氧化物酶（POD）、超氧化物歧化酶（SOD）的活性都在最初的9h内上升较快，且均高于对照，之后开始逐渐下降，丙二醛（MDA）含量也在12h内保持上升，之后略有下降，说明低温胁迫下斑玉蕈细胞膜受到一定伤害，但斑玉蕈对一定范围内的低温有很强的适应性。 |
| 英文摘要: |
| The variation of proteome in mycelium of Hypsizigus marmoreus was analyzed under suitable low temperature by using proteomic methods and techniques for searching the different proteins related closely to cold stress and investigating the function of the proteins. Twenty-six changed protein spots were found under cold stress conditions. Changed protein spots were identified by MALDI-TOF-MS/MS, and fourteen proteins were successfully recognized. In the fourteen differential proteins, actin proteins and tubulin proteins are important components of cytoskeleton; proteasome and acetolactate synthase are involved in the synthesis and decomposition of protein; ATP synthase, transaldolase, formate dehydrogenase and RubisCO are concerned with the metabolize of energy and substance; guanine nucleotide binding protein and 14-3-3 protein participate in control of signal transcription; nascent polypeptide-associated complex may be related to the differentiation and development of cell. It is showed that there may be different kinds of proteins and enzymes involved in the control of Hypsizygus marmoreus in low temperature. The activities of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) raised rapidly in 9 hours of treatment and were higher than those in control, and afterward dropped gradually. Malondialdehyde (MDA) increased in 12h, and later slightly declined, indicating that the cell membrane of Hypsizigus marmoreus was injured. However, Hypsizigus marmoreus possesses strong adaptability in low temperature to a certain extend. |
| 胡开辉 黄桂英 颜松 庄燕煌 危秀芬 朱海潇. 斑玉蕈低温胁迫下菌丝体酶活变化及差异蛋白质组学研究[J]. 菌物学报, 2009, 28(4): 584-590 |
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