| 三种白腐菌及其组合菌种木质素降解酶比较研究 |
| Comparative studies on lignin degradation enzymes produced by three species of white-rot fungi and combination of the strains |
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| 中文关键词:朱红栓菌，糙皮侧耳，黄孢原毛平革菌，酶活 |
| 英文关键词:Trametes cinnabarina, Pleurotus ostreatus, Phanerochaete chrysosporium, enzyme activity |
| 基金项目:教育部“春晖计划”合作科研项目（No. 0221002411015）；重庆大学人才引进基金（No. 0903005104212） |
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| 中文摘要: |
| 朱红栓菌Trametes cinnabarina、糙皮侧耳Pleurotus ostreatus、黄孢原毛平革菌Phanerochaete chrysosporium是产生木质素降解酶能力强的菌株。对三种白腐菌及其组合菌种产生木质素降解酶能力和行为进行了比较分析和研究。结果表明，最佳培养方式为液体振荡培养；最佳培养基为酵母膏液体培养基。在产漆酶（laccases，lacs）方面， Pleurotus ostreatus和Phanerochaete chrysosporium的组合菌种的酶活最强，在第6天出现峰值，酶活达到450U/L；在产锰过氧化物酶（manganese peroxidases，mnps）方面，Trametes cinnabarina 和Pleurotus ostreatus的组合菌种的酶活最强，在第10天出现峰值，酶活达到1050U/L；在产木质素过氧化物酶（lignin peroxidases，lips）方面，Trametes cinnabarina 和Phanerochaete chrysosporium的组合菌种的酶活最强，在第8天出现产酶峰值，酶活达到2990U/L。筛选结果表明，组合菌种比单菌种产生的三种主要木质素降解酶的活性强，这为白腐菌高效产酶提供了一条新的途径，并为白腐菌研究领域的后续工作奠定基础。 |
| 英文摘要: |
| Trametes cinnabarina, Pleurotus ostreatus and Phanerochaete chrysosporium are high-yielding strains producing lignin degradation enzymes. Comparative studies on lignin degradation enzymes produced by these three species of white-rot fungi and combination of the strains were conducted. The results showed that liquid-shaking culture was the best culture method, yeast extract liquid medium was the best medium. As far as laccase (lacs) production was concerned, the enzyme activities of lacs produced by the combination of Pleurotus ostreatus and Phanerochaete chrysosporium were the highest, reaching 450U/L on day 6; the enzyme activities of manganese peroxidases (mnps) produced by the combination of Trametes cinnabarina and Pleurotus ostreatus were the highest, reaching 1050U/L on day 10; the enzyme activities of lignin peroxidases (lips) produced by the combination of Trametes cinnabarina and Phanerochaete chrysosporium were the highest, reaching 2990U/L on day 8. The activities of three kinds of main lignin degradation enzymes produced by combination of the three species of white-rot fungi were much higher than those produced by single strain of the fungi. It is suggested that combined strains might be effective for increasing the yield of enzyme production. |
| 段传人 朱丽平 姚月良. 三种白腐菌及其组合菌种木质素降解酶比较研究[J]. 菌物学报, 2009, 28(4): 577-583 |
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