| 寄生隐丛赤壳菌Cryphonectria parasitica致病毒素Cp-I的分离纯化和结构分析 |
| Isolation, purification and structure of Cp-I toxin from Cryphonectria parasitica |
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| 中文关键词:质谱，核磁共振氢谱，核磁共振碳谱，红外光谱 |
| 英文关键词:mass spectrography, nuclear magnetic resonance, infrared spectrum |
| 基金项目:四川省学术和技术带头人培养基金；国家自然科技资源共享平台项目（No. 2005DKA21207-13） |
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| 中文摘要: |
| Cryphonectria parasitica 菌株经液体培养，石油醚萃取其发酵液获得对板栗带叶嫩枝具有致萎活性的粗提物，以氯仿:石油醚:甲醇（6:2:2）作洗脱剂，粗提物经硅胶色谱分离，共得到3组纯组份，其中第1组份（Cp-I）对板栗幼苗致萎活性较高。质谱、核磁共振和红外光谱测定表明Cp-I分子量为278，化学式为C16H22O4。 |
| 英文摘要: |
| The strain of Cryphonectria parasitica was cultivated by liquid fermentation. The crude material with wilt-inducing activity to chestnut browse was obtained by using petroleum ether extracting method from the fermentation broth. Three compounds were obtained by means of silica gel column chromatography using mixed solution of V(chloroform ):V(petroleum ether ):V(alcohol) = 6:2:2 as eluent. The results showed the first compound (Cp-I) had higher wilt-inducing activity than the other two. The chemical formula of the pathogenic fraction Cp-I was identified as C16H22O4 (M=278) by mass spectrography (MS), nuclear magnetic resonance (NMR) and infrared spectrum (IR). |
| 韩珊 朱天辉. 寄生隐丛赤壳菌Cryphonectria parasitica致病毒素Cp-I的分离纯化和结构分析[J]. 菌物学报, 2009, 28(4): 535-540 |
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