| 裂蹄木层孔菌子实体水提物诱导HepG2细胞凋亡的初步研究 |
| Water extract of Phellinus linteus fruiting body induces apoptosis of HepG2 cells |
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| 中文关键词:大型真菌，提取物，抗肿瘤，Bax，Bcl-2 |
| 英文关键词:macrofungi, extract, antitumor, Bax, Bcl-2 |
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| 中文摘要: |
| 研究裂蹄木层孔菌子实体水提物（WEPL）对人类克隆肝癌细胞系HepG2生长的作用。用裂蹄木层孔菌子实体水提物处理HepG2细胞后，噻唑蓝法（MTT法）可见浓度和时间依赖性抑制细胞增殖；电镜下观察凋亡小体的出现，流式细胞仪技术显示Annexin-Ⅴ染色呈阳性，都证明了HepG2细胞发生了凋亡。RT-PCR和Western Blot分析证实WEPL刺激Bax表达量上调、Bcl-2表达量下调进而诱导了细胞凋亡。结果表明WEPL诱发的克隆人类肝癌细胞系HepG2的细胞凋亡可能是通过上调Bax、下调Bcl-2活性来实现的。 |
| 英文摘要: |
| The effect of water extracts of Phellinus linteus fruit bodies (WEPL) on the growth of HepG2 human colon cancer cells was investigated. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay showed that the inhibition ratio of HepG2 cells treated with WEPL increased dose- and time- dependently. Microscopic observation proved the occurrence of apoptotic bodies. Flow cytometry detection showed the cells were positively stained by Annexin-Ⅴ. These were the hallmark features of apoptosis. The mRNA expression level and protein level of Bax were adjusted up while that of Bcl-2 were adjusted down. It seems possible that WEPL induces HepG2 cell apoptosis through down-regulating the expression of Bcl-2 gene and up-regulating the expression of Bax gene. |
| 董露璐 赵敏 安晓丽 何平平 相文华. 裂蹄木层孔菌子实体水提物诱导HepG2细胞凋亡的初步研究[J]. 菌物学报, 2009, 28(3): 451-455 |
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