| 利用木霉与根霉两步发酵秸秆制备L-乳酸研究 |
| Two-step fermentation of crop straw by using Trichoderma viride and Rhizopus oryzae for producing L-lactic acid |
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| 中文关键词:绿色木霉，米根霉，发酵工艺 |
| 英文关键词:Trichoderma viride, Rhizopus oryzae, fermentation technics |
| 基金项目:国家科技支撑计划（No. 2007BAD34B01）；安徽省自然科学基金（No. 070411024）；合肥工业大学基金（No. 073002F） |
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| 中文摘要: |
| 以秸秆为原料进行生物转化大量制备有机酸意义重大。在秸秆汽爆法预处理的基础上，以绿色木霉为菌种转化制备秸秆糖，对降解单糖接种米根霉进行二次发酵制备L-乳酸。试验结果表明，第一步绿色木霉固态培养制备纤维素酶时，控温30℃、通气0.12L/(L?min)、发酵40h后制备干曲，后按10g干曲/L汽爆液的配比进行55℃酶解36h，五、六碳糖累积浓度达到86g/L。第二步米根霉发酵时，控制温度32℃、通气0.4L/(L?min)、转速450r/min，发酵48h，最终产L-乳酸累积浓度为81.6g/L。秸秆制备L-乳酸的两步发酵法发酵工艺具有推广价值。 |
| 英文摘要: |
| Crop straw was pretreated by steam-exploded method, and Trichoderma viride was used for degradation of crop straw in the first-step fermentation, then Rhizopus oryzae was used for producing L-lactic acid in the second-step fermentation. The optimal condition for the first-step fermentation was as follows: fermentation temperature of 30℃, aeration rate of 0.12L/(L?min) for 40 hours, including pentose and hexose sugar concentration of about 86g/L when 10g dry biomass from the first-step fermentation was used for the degradation of 1L hydrolyte. The yield of L-lactic acid was about 81.6g/L when the second-step fermentation condition was as follows: temperature of 32℃, aeration rate of 0.4L/(L?min), rotation rate of 450r/min and fermentation period of 48 hours. It was concluded that the two-step fermentation technics for the production of L-lactic acid from crop straw was worthy to be developed on a large scale. |
| 李兴江 郑志 姜绍通 潘丽军. 利用木霉与根霉两步发酵秸秆制备L-乳酸研究[J]. 菌物学报, 2009, 28(3): 445-450 |
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