| 毛木耳Auricularia polytricha多糖APPIIA对巨噬细胞细胞因子和iNOS基因表达的影响 |
| Effects of Auricularia polytricha polysaccharide on mouse macrophage cytokine and iNOS gene expression |
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| 中文关键词:食药用菌，小鼠，巨噬细胞，抗肿瘤，一氧化氮 |
| 英文关键词:edible and medicinal fungus, mouse macrophages, anti-tumor, nitric oxide |
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| 中文摘要: |
| 从毛木耳Auricularia polytricha子实体中分离得到了具有抗肿瘤作用的单一多糖组分APPIIA，采用SYBR Green I指示的Real-time RT-PCR技术研究了它对小鼠巨噬细胞RAW 264.7中IL-1β，IL-6，TNF-α和一氧化氮（NO）合成关键酶iNOS基因在mRNA转录水平上的作用。采用Western-Blot技术，研究了APPIIA对RAW 264.7细胞生成iNOS蛋白的能力。研究发现，APPIIA能增强IL-1β，IL-6，TNF-α和NO合成关键酶iNOS基因的转录水平，并能增加iNOS蛋白的生成。推测APPIIA可能通过上调相关基因的表达，增加IL-1β，IL-6，TNF-α分泌和NO生成，从而实现其抗肿瘤的重要作用。 |
| 英文摘要: |
| The gene transcription level of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and iNOS in RAW 264.7 was detected by real-time RT-PCR method. The protein expression level of iNOS was also tested by Western-Blot method. It was found that the transcribe level of these genes was all enhanced by Auricularia polytricha polysaccharide APPIIA in a dose-dependent manner and the protein level of iNOS was also enhanced by APPIIA. It could be concluded that APPIIA might promote the expression of these genes and inhibit the tumor markedly. |
| 罗霞 余梦瑶 江南 许晓燕 曾瑾 郑林用. 毛木耳Auricularia polytricha多糖APPIIA对巨噬细胞细胞因子和iNOS基因表达的影响[J]. 菌物学报, 2009, 28(3): 435-439 |
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