| 黑曲霉变异株A-828酸性植酸酶的生物合成和性质的研究 |
| The biological synthesis, purification and characterization of an acid phytase from Aspergillus niger A-828 |
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| 中文关键词:纯化，原生质体，PhyB |
| 英文关键词:purification, protoplast, phytase |
| 基金项目:国家科技攻关计划（No. 96-03-02-02） |
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| 中文摘要: |
| 经钴60及原生质体紫外联合诱变后，筛选出一株高产植酸酶的菌株（A-828），酶活力达到66,000U/mL，为出发株的17倍。通过滤膜超滤浓缩和Bio-gel P-150层析纯化，纯化倍数为11.2倍，活力回收率为37.7%。SDS-PAGE分析表明，变异株植酸酶的分子量约为66kDa，酶学实验结果表明：该酶的最适pH有两个，分别为2.5和 5.5，但在2.5时所表现的活力是5.5时的5倍，酶的最适温度为55℃。在20－60℃保温20min，活力不改变。2mmol/L的Fe2+、Cu 2+、Cr3+抑制了酶的活性，但同样浓度Ca 2+、Mn 2+、EDTA、DTT对酶的活力影响不显著。 |
| 英文摘要: |
| Screening out a novel Aspergillus niger strain (A-828) which expresses high active acid phytase through combination mutation by Co-60 and UV. To research its enzymatic characterization, the acid phytase was purified by the steps of ultrafiltration and Bio-gel-P-150 chromatography. The purification ratio and yield was 11.2 and 29%. Result shows that its molecular weight was estimated to be about 66kDa by SDS-PAGE. The phytase activity of A-828 reached to the 66,000U/mL, 17 fold than origin. Its optimum pH of the enzyme activity was both at 2.5 and 5.5, but the largest activity at 2.5 was 5 fold than that at 5.5. Its optimum temperature was 55℃. The thermal stability of the phytase could be unchanged during 20℃ to 60℃ for 20 min. The phytase activity was strongly inhibited by 2mmol/L Cu2+ , Fe2+ and Cr2+, while could not be influence by Ca2+, Mn2+, EDTA, DTT. |
| 曾继蛟 李英伦 王鸿宾 石峰 岳丽丽 李运敏 刘志刚 矫庆华. 黑曲霉变异株A-828酸性植酸酶的生物合成和性质的研究[J]. 菌物学报, 2009, 28(3): 428-434 |
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