| 食药用菌功能性β-葡聚糖荧光法测定研究 |
| Fluorometry method for quantitative determination of functional β-glucans from edible and medicinal mushrooms |
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| 中文关键词:酵母，β-1,3/1,6-葡聚糖，苯胺蓝，酶法验证 |
| 英文关键词:yeast, β-1,3/1,6-glucan, aniline blue, validation using enzyme method |
| 基金项目:浙江省重大专项重点项目（No. 2006C12048） |
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| 中文摘要: |
| 以酵母功能性β-1,3/1,6-葡聚糖为对照品，利用苯胺蓝和β-1,3/1,6-葡聚糖特异结合荧光特性，研究了葡聚糖荧光法测定时的各影响因素，建立了荧光法测定食药用菌功能性β-葡聚糖的方法。pH9.6缓冲液，80℃条件下避光反应15min，室温30min冷却后，398nm激发波长，508nm发射波长，20℃下进行荧光测定。在测定浓度2－20μg/mL范围内，荧光强度与浓度具有良好的线性关系（R2=0.9977），其中检出限为45μg/L，测定精密度和加样回收率良好，相对标准偏差（RSD）分别为1.86%和3.40%，并与酶法进行了比对验证，一致性良好，且荧光法更为节约时间和成本，并对灰树花菌、巴氏蘑菇、香菇和鲍氏针层孔菌四种食药用菌β-葡聚糖提取样品进行了葡聚糖纯度和提取率测定。 |
| 英文摘要: |
| Based on the fluorescent property of specific integration of aniline blue dye and standard functional yeast β-1,3/1,6-glucan, some influencing factors of glucan fluorometry were investigated and β-glucan fluorescent determination method was developed. Under the conditions of buffer solution of pH 9.6, aniline blue reaction on yeast β-1,3/1,6-glucan at 80℃ for 15min in dark, then cooling at room temperature for 30min and fluorescent analysis at 20℃ with an excitation maximum wavelength of 398nm and an emission maximum wavelength of 508nm, the standard curve had a good linearity with a concentration range of 2－20μg/mL (R2=0.9977), and the detectable limit was 45μg/L. The precision and recovery of fluorescent method were both measured with a relative standard deviation (RSD) as 1.86% and 3.40%. The method was also validated by enzyme method and showed preferable time saving and economization of cost. The purity and yield of extracted β-glucans samples of Grifola frondosa, Agaricus blazei, Lentinula edodes and Phellinus baumii were analyzed by the method. |
| 杨开 胡君荣 何荣军 张安强 吴学谦 孙培龙. 食药用菌功能性β-葡聚糖荧光法测定研究[J]. 菌物学报, 2009, 28(3): 399-406 |
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