| 中华羊茅内生真菌Neotyphodium sp.生物学与生理学特性的研究 |
| Biological and physiological characteristics of Neotyphodium endophyte symbiotic with Festuca sinensis |
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| 中文关键词:共生体，碳源和氮源，生长温度，培养基 |
| 英文关键词:symbiont, carbon and nitrogen source, growing temperature, cultural medium |
| 基金项目:国家973课题（No. 2007CB108902）；国家自然科学基金（No. 30771531，30070546） |
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| 中文摘要: |
| 本文对中华羊茅Festuca sinensis内生真菌Neotyphodium sp.的生物学与生理学特性进行了报道。研究发现：中华羊茅内生真菌能在10－30℃生长，5℃和35℃几乎不能生长，最适生长温度是25℃；适宜的pH是7－9；不同的碳、氮源利用能力不同，利用能力最好的分别是甘露醇和酵母浸液；在不同培养基上的生长速度与产孢特性存在差异，马铃薯葡萄糖琼脂（PDA）培养基上生长最快，水琼脂（WA）和海水营养琼脂（SNA）培养基上生长最慢，但WA和SNA产孢最多。 |
| 英文摘要: |
| Biological and physiological characteristics of Neotyphodium endophyte from Festuca sinensis were studied. The results showed that Neotyphodium sp. endophyte was able to grow at 10–30℃, but almost not at 5℃ and 35℃. The optimal temperature and pH values for mycelial growth of the endophyte was 25℃ and 7–9, respectively. The endophyte varied in their viability on media containing different carbon and nitrogen nutrients. The preference of endophyte for carbon and nitrogen source was mannitol and yeast extract, respectively. The endophyte mycelium grew significantly better on potato dextrose agar (PDA) than on oat meal agar (OMA), corn meal agar (CMA) and F. sinensis meal agar (FMA), and most slowly on water agar (WA) and saltwater nutrient agar (SNA)．Sporulation of the endophyte on WA and SNA was better than that on others. |
| 金文进 李春杰 南志标. 中华羊茅内生真菌Neotyphodium sp.生物学与生理学特性的研究[J]. 菌物学报, 2009, 28(3): 363-369 |
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