| 一株来自于伊犁贝母的内生尖孢镰孢菌Y1的抑菌活性初步研究 |
| Antibacterial activity of an endophytic fungus, Fusarium oxysporum Y1 isolated from the bulbs of Fritillaria pallidiflora |
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| 中文关键词:病原真菌，镰孢菌属，乙酸乙酯浸膏，正丁醇浸膏，最低抑菌浓度 |
| 英文关键词:pathogenic fungi, Fusarium, acetic ether extract, n-Butanol extract, minimum inhibitory concentration |
| 基金项目:教育部高等学校科技创新工程重大项目培育资金项目（No. 130105） |
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| 中文摘要: |
| 从新疆巩留县伊犁贝母的新鲜鳞茎中分离到一株具有分泌抑菌活性物质的内生尖孢镰孢菌Fusarium oxysporum Y1，该菌在7种不同培养基上生长时显示出不同的菌落生长特征，而且只在沙氏培养基中生长时才具有分泌抑菌活性物质的能力。抑菌活性筛选结果表明：由该菌及其发酵液制备的发酵液浸膏、菌体裂解液浸膏以及经进一步纯化后获得的乙酸乙酯浸膏和正丁醇浸膏均具有明显的抑菌活性，其中以发酵液的乙酸乙酯浸膏和菌体裂解液的正丁醇浸膏活性最强，它们对金黄色葡萄球菌Staphylococcus aureus、表皮葡萄球菌Staphylococcus epidermidis、枯草芽孢杆菌Bacillus subtilis、藤黄八叠球菌Sarcina lutea和大肠杆菌Escherichia coli的最低抑菌浓度均小于25μg/mL。 |
| 英文摘要: |
| An endophytic fungus, Fusarium oxysporum Y1 was isolated from the fresh bulbs of Fritillaria pallidiflora Schrenk in Gongliu county, Xinjiang Uygur Autonomous Region of China. The fungus showed variable colony features when it was cultured on different media, and could secrete antibacterial metabolites in sabouraud’s dextrose agar (SDA) liquid medium. Further analysis showed all the fractions extracted from the fermentation broth and mycelia had abilities to suppress the growth of bacteria. The acetic ether extract from the fermentation broth and n-butanol extract from the mycelial lysates showed the strongest inhibitions against the growth of Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Sarcina lutea and Escherichia coli with the minimum inhibitory concentrations (MIC) lower than 25μg/mL. |
| 祝兴伟 张少华 王治维 李萍 叶波平. 一株来自于伊犁贝母的内生尖孢镰孢菌Y1的抑菌活性初步研究[J]. 菌物学报, 2009, 28(3): 349-353 |
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