| 茶树内生木霉种的鉴定及其在植物体内的定殖 |
| An endophytic Trichoderma species from Camellia sinensis: its characterization and endophytism |
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| 中文关键词:长枝木霉，人工接种，定殖 |
| 英文关键词:Trichoderma longibrachiatum, artificial inoculation, colonization |
| 基金项目:福建省自然科学基金重点项目（No. B0620005） |
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| 中文摘要: |
| 采用分离自健康茶树叶片组织的一株长枝木霉Trichoderma longibrachiatum 菌株CSN-18，研究茶树内生木霉的人工接种、再分离及其在茶树地上部组织中的内生性。该菌在PDA培养基上生长速度快，产孢量大。用CSN-18回接茶苗，接种后一个月可以从茶苗的茎和叶组织中再分离获得该真菌。接种后的茶苗没有观察到明显的病害表现；与对照相比，接种后6个月组培苗生长良好，叶色更绿；接种后1个月，实生苗生长正常。通过石蜡切片和苯胺蓝染色，在已接种的茶树组培苗的叶片内部组织中可观察到木霉的存在，从而证明了木霉能够在茶树地上部组织内定殖，是茶树的一种内生真菌。 |
| 英文摘要: |
| Fourteen strains of endophytic Trichoderma were isolated from healthy tea plant by using conventional isolation method. The strain Trichoderma longibrachiatum CSN-18 was used as inoculum for studying its endophytism in tea seedlings. The strain grew quickly in PDA media and yielded masses of conidiospores. After being inoculated to tea seedlings, the endophytic Trichoderma longibrachiatum CSN-18 was re-isolated from the seedlings in 30 days. The tea seedlings inoculated with Trichoderma longibrachiatum CSN-18 did not show any disease symptom, and the tissue-cultured seedlings grew better than un-inoculated seedlings in 6 months. The presence of the fungus in the leaf tissues was studied by means of paraffin-cut section dyed with aniline blue. The results showed that the fungus was observed on the surface and in inner tissues of leaves, indicating that Trichoderma longibrachiatum strain CSN-18 could establish colonization in ground portion of tea seedlings as an endophyte. |
| 武汉琴 苏经迁 谢明英 杨民和. 茶树内生木霉种的鉴定及其在植物体内的定殖[J]. 菌物学报, 2009, 28(3): 342-348 |
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