| 球孢白僵菌热休克蛋白基因Bbhsp70的cDNA及上游序列克隆与分析 |
| Cloning and sequence analysis of cDNA and upstream sequence of Bbhsp70 gene from Beauveria bassiana |
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| 中文关键词:昆虫病原真菌，RACE，DNA步移，上游调控序列 |
| 英文关键词:entomopathogenic fungi, RACE, DNA walking, upstream regulation sequence |
| 基金项目:国家“863”计划（No. 2006AA10A212）；新世纪优秀人才支持计划（No. 05-0560）；安徽省优秀青年科技基金（No. 08040106902）；安徽省级自然科学研究重点项目（No. TD200708） |
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| 中文摘要: |
| 运用SMART RACE RT-PCR技术与DNA步移技术，首次从球孢白僵菌中克隆出完整的热休克蛋白基因Bbhsp70编码区序列及上游序列。该基因cDNA全长2405bp，5′端非翻译区171bp，3′端非翻译区263bp，开放阅读框（ORF）1971bp，编码656个氨基酸。成熟蛋白理论分子量为71.3kDa，理论等电点为4.92。上游序列长度3559bp，其中有305bp序列与cDNA序列重叠。分析表明，上游序列中没有明显的TATA-盒和CAAT-盒，但含有CCAAT-binding factor、GC-box等重要的转录因子结合位点，以及热激应答元件（HSE）和GATA元件等启动子顺式调控元件。 |
| 英文摘要: |
| The full-length cDNA of Bbhsp70 gene was cloned from Beauveria bassiana using SMART RACE RT-PCR, and its upstream sequence was amplified using genome walking. Analysis of the cloned complete cDNA with a whole length of 2405bp showed that it encompassed a open reading frame (ORF) with 1971bp encoding 656 amino acid. The mature protein had a molecular mass of 71.3kDa with a calculated pI of 4.92. The analyses indicate that the upstream sequence contained several regulatory elements, such as CCAAT-binding factor, GC-box, HSE and GATA, but the TATA-box and CAAT-box could not be found. The findings of this study are essential for further functional investigation of hsp70 gene of Beauveria bassiana and construction of engineered strain with enhanced anti-adversity ability. |
| 谢 翎 陈红梅 蒲顺昌 汤 强 李增智 黄 勃. 球孢白僵菌热休克蛋白基因Bbhsp70的cDNA及上游序列克隆与分析[J]. 菌物学报, 2009, 28(2): 283-288 |
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